ArticlePloS one2018
Effects of water decontamination methods and bedding material on the gut microbiota.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Interlaboratory clinical chemistry parameter variation in seven-day acute hydrazine toxicity studies in the Sprague-Dawley rat.Archives of toxicology · 2026Article
- Beyond Comfort: A Review of the Critical Role of Bedding Selection in Rodent Welfare, Microenvironment, and Research Reproducibility.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Review
- Gut microbiota and pathobiont exposure influences disease incidence in non-obese diabetic mice.Frontiers in microbiology · 2026Article
- Dominant effects of the immediate environment on the gut microbiome of mice used in biomedical research.mSystems · 2025Article
- Role of gut microbiota and its functional products in prenatal alcohol-induced anxiety-like behavior.Progress in neuro-psychopharmacology & biological psychiatry · 2025Article
- Influence of Water Delivery Method on the Gut Microbiome in Laboratory Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Effects of antibiotic cocktail on the fecal microbiota and their potential correlation of local immune response.BMC microbiology · 2024Article
- Transgenerational effects of early life stress on the fecal microbiota in mice.Communications biology · 2024Article
- Vivaria housing conditions expose sex differences in brain oxidation, microglial activation, and immune system states in aged hAPOE4 mice.Experimental brain research · 2024Article
- Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight.Cell reports · 2023Article
- Effects of Extended Cage Component Sanitation Interval on the Microenvironment, Health, and Gastrointestinal Microbiome of Rats (Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Effect of Husbandry Practices on the Fecal Microbiota of C57BL/6J Breeding Colonies Housed in 2 Different Barrier Facilities in the Same Institution.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Review
- Reduced housing density improves statistical power of murine gut microbiota studies.Cell reports · 2022Article
- Deep Learning Encoding for Rapid Sequence Identification on Microbiome Data.Frontiers in bioinformatics · 2022Article
- Cocaine-Induced Locomotor Activation Differs Across Inbred Mouse Substrains.Frontiers in psychiatry · 2022Article
- Evaluation of Effects of Laboratory Disinfectants on Mouse Gut Microbiota.Comparative medicine · 2021Article
- The gut microbiome of laboratory mice: considerations and best practices for translational research.Mammalian genome : official journal of the International Mammalian Genome Society · 2021Review
- Weight Gain, Glucose Tolerance, and the Gut Microbiome of Male C57BL/6J Mice Housed on Corncob or Paper Bedding and Fed Normal or High-Fat Diet.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Shared and distinctive features of the gut microbiome of C57BL/6 mice from different vendors and production sites, and in response to a new vivarium.Lab animal · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Rodent models are invaluable to understanding health and disease in many areas of biomedical research. Unfortunately, many models suffer from lack of phenotype reproducibility. Our laboratory has shown that differences in gut microbiota (GM) can modulate phenotypes of models of colon cancer and inflammatory bowel disease. We and others have also shown that a number of factors associated with rodent research, including vendor, cage system, and bedding can alter GM. The objective of this study was to expand these studies to examine the effect of additional bedding materials and methods of water decontamination on GM diversity and composition. To this end, Crl:CD1 (ICR) mice were housed on corn cob or compressed paper chip bedding and provided water that was decontaminated by four different methods: autoclaving with reverse osmosis, autoclaving with hydrochloric acid, autoclaving with sulfuric acid, and autoclaving alone. Feces was collected at day 0, and at day 28 (endpoint), fecal and cecal samples were collected. DNA was extracted from samples, amplified by PCR using conserved bacterial primer sets and subjected to next generation sequencing. Sequence data were analyzed using Qiime and groups were compared using principal coordinate analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA). Two factor PERMANOVA of cecal GM data revealed significant changes when comparing bedding and water decontamination methods, while no significant effects were noted in the fecal GM data. Subsequent PERMANOVA and PCoA of cecal data revealed that several combinations of bedding and water decontamination methods resulted in differing GM, highlighting the complexity by which environmental factors interact to modulate GM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.