ArticleStem cell research & therapy2018
Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity.
Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- Pooled it
- Article
- Mouse Trophoblast Stem Cells Are Deficient in TNFα-Activated NFκB Signaling Pathway and Inflammatory Response.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Article
- Attenuated innate immunity in embryonic stem cells: mechanisms and therapeutic applications.Cell & bioscience · 2025Review
- A Prominent Pro-Inflammatory Phenotype Is Observed in Replication and Stress-Induced Senescent Mast Cells.Aging cell · 2025Article
- Mouse Trophoblast Cells Have Attenuated Responses to TNF-α and IFN-γ and Can Avoid Synergic Cytotoxicity of the Two Cytokines.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Allogeneic fibroblasts ameliorate intervertebral disc degeneration by reducing osteophytes in rabbits.Frontiers in medicine · 2024Article
- Equine induced pluripotent stem cells are responsive to inflammatory cytokines before and after differentiation into musculoskeletal cell types.In vitro cellular & developmental biology. Animal · 2023Article
- Mouse Trophoblast Cells Can Provide IFN-Based Antiviral Protection to Embryonic Stem Cells via Paracrine Signaling.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine.Materials today. Bio · 2022Article
- Dicer and PKR as Novel Regulators of Embryonic Stem Cell Fate and Antiviral Innate Immunity.Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- Establishment, characterization, and validation of novel porcine embryonic fibroblasts as a potential source for genetic modification.Frontiers in cell and developmental biology · 2022Article
- Novel function ofIranian journal of basic medical sciences · 2021Article
- Pluripotent stem cells are insensitive to the cytotoxicity of TNFα and IFNγ.Reproduction (Cambridge, England) · 2020Article
- The involving progress of MSCs based therapy in atherosclerosis.Stem cell research & therapy · 2020Review
- Epigenetic regulation of IFITM1 expression in lipopolysaccharide-stimulated human mesenchymal stromal cells.Stem cell research & therapy · 2020Article
- The underdeveloped innate immunity in embryonic stem cells: The molecular basis and biological perspectives from early embryogenesis.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Dicer represses the interferon response and the double-stranded RNA-activated protein kinase pathway in mouse embryonic stem cells.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMesenchymal stem cells (MSCs) isolated from adult tissues (Ad-MSCs) have shown great promise for use in regenerative medicine. However, their poor in vitro expansion capacity and tissue scarcity have been major limitations. In this study, we demonstrate that mouse embryonic stem cells (mESCs) can differentiate into cells with MSC properties.
methodsUsing previously established methods that characterize Ad-MSCs, we analyzed mESC-differentiated fibroblasts (mESC-FBs), including plastic adherence, clonogenic growth, MSC marker expression, tri-lineage differentiation potential, and the capacity to express immunomodulators.
resultsAlthough previously characterized as mESC-differentiated fibroblasts (mESC-FBs), these cells exhibit major properties of Ad-MSCs. However, mESC-FBs also display unique features inherited from ESCs, including robust expansion capacity, senescence resistance, and attenuated innate immunity. In particular, mESC-FBs are insensitive to bacterial endotoxin (lipopolysaccharide, LPS) and do not express LPS-induced inflammatory molecules, in contrast to bone marrow (BM)-MSCs. We further demonstrate that mESC-FBs are resistant to the cytotoxicity associated with inflammatory cytokines, bacterial endotoxins (LPS and heat-killed bacteria), and macrophage-mediated inflammation.
conclusionsWhile it remains to be determined how the unique properties of mESC-FBs will affect their immunoregulatory activity under an in vivo condition, our findings demonstrate that ESCs could be used as an alternative source to generate a new class of ESC-MSCs with unique features potentially useful in regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.