Evidence map›Paper›PMID 30359279›Full record

ArticleMicrobial cell factories2018

Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region.

Lin Liu, Haoran Yu, Kun Du, Zhiyan Wang, Yiru Gan, He Huang

Abstract read
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In one paragraph

Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Enhancement of the Thermostability ofMolecules (Basel, Switzerland) · 2024
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lin LiuDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China.
Haoran YuDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China.
Kun DuDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China.
Zhiyan WangDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China.
Yiru GanDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China.
He HuangDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China. huang@tju.edu.cn.

Funding

National Science and Technology Major Projects of China 2014ZX09508006-002-002
6 · The paper itself

Abstract

backgroundHigh thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein thermostability.

resultsThe flexible regions in porcine trypsin were predicted using the methods including molecular dynamic simulation, FlexPred, and FoldUnfold. The amino acids 78-90 was predicted to be the highly flexible region simultaneously by the three methods and hence selected to be the mutation target. We constructed five variants (D3, D5, D7, D9, and D11) by truncating the region. And the variant D9 showed higher thermostability, with a 5 °C increase in T

conclusionsThese results showed that the thermostability of variant D9 was increased. The variant D9 could be expected to be a promising tool enzyme for its wider industrial applications. The method of truncating the flexible region used in our study has the potential to be used for enhancing the thermostability of other proteins.

Indexed as

TemperatureAnimalsCircular DichroismEnzyme StabilityKineticsMutant ProteinsMutationPichiaSpectrometry, FluorescenceSus scrofaTrypsinMutant ProteinsTrypsinFlexible regionThermostabilityTruncationTrypsinΩ-loop

Identifiers

PMID30359279
PMCPMC6201580

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.