ArticleJournal of translational medicine2018
iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals.
Article in Journal of translational medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Potential of Salivary Biomarkers in Autism Research: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Association of salivary proteins with periodontal disease in children and adolescents- a scoping review.BMC oral health · 2026Article
- Convergent musk biosynthesis across host and microbiota in musk deer and muskrat.Zoological research · 2025Article
- The advancement of nanosystems for drug delivery in the prevention and treatment of dental caries.Frontiers in cellular and infection microbiology · 2025Review
- Starch treatment improves the salivary proteome for subject identification purposes.Forensic science, medicine, and pathology · 2024Article
- Encapsulation of a novel peptide derived from histatin-1 in liposomes against initial enamel caries in vitro and in vivo.Clinical oral investigations · 2023Article
- Biomarkers of aging.Science China. Life sciences · 2023Review
- Comparison of oral cavity protein abundance among caries-free and caries-affected individuals-a systematic review and meta-analysis.Frontiers in oral health · 2023Review
- Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma.Liver international : official journal of the International Association for the Study of the Liver · 2022Article
- iTRAQ-based proteomic analysis of differentially expressed proteins in sera of seronegative and seropositive rheumatoid arthritis patients.Journal of clinical laboratory analysis · 2022Article
- Overview of methods for characterization and visualization of a protein-protein interaction network in a multi-omics integration context.Frontiers in molecular biosciences · 2022Review
- The salivary proteome reflects some traits of dietary habits in diabetic and non-diabetic older adults.European journal of nutrition · 2021Article
- Clinical Proteomics of Biofluids in Haematological Malignancies.International journal of molecular sciences · 2021Review
- Downregulation of Salivary Proteins, Protective against Dental Caries, in Type 1 Diabetes.Proteomes · 2021Article
- Review
- The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years.BMC oral health · 2020Article
- Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3.International journal of molecular sciences · 2020Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHuman saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with aging of patients with dental caries. Here, we applied isobaric tags for relative and absolute quantitation (iTRAQ) in combination with multiple reaction monitoring mass spectrometry (MRM-MS) to characterize the salivary proteome profiles of subjects of different ages, presenting with and without caries, with the aim of identifying age-related biomarkers for dental caries.
methodsUnstimulated whole saliva samples were collected from 40 caries-free and caries-susceptible young adults and elderly individuals. Salivary proteins were extracted, reduced, alkylated, digested with trypsin and then analyzed using iTRAQ-coupled LC-MS/MS, followed by GO annotation, biological pathway analysis, hierarchical clustering analysis, and protein-protein interaction analysis. Candidate verification was then conducted using MRM-MS.
resultsAmong 658 salivary proteins identified using tandem mass spectrometry, 435 proteins exhibited altered expression patterns in different age groups with and without caries. Of these proteins, 96 displayed age-specific changes among caries-susceptible adults and elderly individuals, and were mainly associated with salivary secretion pathway, while 110 age-specific proteins were identified among healthy individuals. It was found that the age factor caused significant variations and played an important role in both healthy and cariogenic salivary proteomes. Subsequently, a total of 136 target proteins with complex protein-protein interactions, including 14 age-specific proteins associated with caries, were further successfully validated using MRM analysis. Moreover, non-age-specific proteins (histatin-1 and BPI fold-containing family B member 1) were verified to be important candidate biomarkers for common dental caries.
conclusionsOur proteomic analysis performed using the discovery-through-verification pipeline revealed distinct variations caused by age factor in both healthy and cariogenic salivary proteomes, highlighting the significance of age in the great potential of saliva for caries diagnosis and biomarker discovery.
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