ArticleHistochemistry and cell biology2019
Characteristics of the tissue section that influence the staining outcome in immunohistochemistry.
Article in Histochemistry and cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
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- An interpretable framework for gastric cancer classification using multi-channel attention mechanisms and transfer learning approach on histopathology images.Scientific reports · 2025Article
- Impact of ALDH1A1 Expression in Intrahepatic Cholangiocellular Carcinoma.Journal of Cancer · 2025Article
- Enhancing AI Research for Breast Cancer: A Comprehensive Review of Tumor-Infiltrating Lymphocyte Datasets.Journal of imaging informatics in medicine · 2024Review
- An Optimized and Advanced Algorithm for the Quantification of Immunohistochemical Biomarkers in Keratinocytes.JID innovations : skin science from molecules to population health · 2024Article
- Viruses in glioblastoma: an update on evidence and clinical trials.BJC reports · 2024Review
- Collagen type XII is undetectable in keratoconus Bowman's layer.The British journal of ophthalmology · 2024Article
- The Compartmentalization of Amyloid-β in Idiopathic Normal Pressure Hydrocephalus Brain Biopsies.Journal of Alzheimer's disease : JAD · 2024Article
- Impact of formalin fixation on mismatch repair protein evaluation by immunohistochemistry.Virchows Archiv : an international journal of pathology · 2023Article
- The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors.Diagnostics (Basel, Switzerland) · 2023Article
- Methylation differences in Alzheimer's disease neuropathologic change in the aged human brain.Acta neuropathologica communications · 2022Article
- Cell2Grid: an efficient, spatial, and convolutional neural network-ready representation of cell segmentation data.Journal of medical imaging (Bellingham, Wash.) · 2022Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunohistochemistry (IHC) is influenced by several factors such as cold ischemia time, fixative, fixation time, paraffin, storage time, antibody, antigen retrieval technique and detection systems. In the setting of post-mortem tissue, not only post-mortem delay, but also agonal state is of interest. Here, we assessed an additional variable, i.e., the thickness of the section, and noted that this variable also influenced the IHC outcome. This is of significance when the extent of labelling is a parameter to be assessed, for example when assigning a stage or grade of a disease. Furthermore, when assessing brain tissue with neurons, soma measuring from 4 to 100 µm, various cellular compartments composed of different proteins are localised in sections measuring 4 or 7 µm. Thus, what is seen in a 7-µm-thick section might be lacking in a 4-µm-thick section. Lack of information regarding the molecular size of commercial antibodies is also disturbing as this parameter might influence the distribution of the molecule in the three-dimensional section. The choice of antibody to be used and the staining methodology have been acknowledged being of significance for IHC outcome; however, neither sections thickness or the molecular weight has been discussed sufficiently. IHC has been shown to be an unpredictable technique used for assessment of tissue. This emphasises the need for detailed methodological descriptions in publications, the need to acknowledge and to harmonize all eventual pitfalls related to this methodology.
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