ArticleNucleic acids research2018
Single-cell stabilization method identifies gonadotrope transcriptional dynamics and pituitary cell type heterogeneity.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Single-Cell RNA Sequencing and Its Applications in Pituitary Research.Neuroendocrinology · 2024Review
- Sex-Linked Growth Disorder and Aberrant Pituitary Gene Expression in Nestin-Cre-MediatedBiology · 2023Article
- A Simple Evolutionary Model of Genetic Robustness After Gene Duplication.Journal of molecular evolution · 2022Article
- Article
- Single nucleus multi-omics regulatory landscape of the murine pituitary.Nature communications · 2021Article
- Cryopreservation of microglia enables single-cell RNA sequencing with minimal effects on disease-related gene expression patterns.iScience · 2021Article
- Versatile workflow for cell type-resolved transcriptional and epigenetic profiles from cryopreserved human lung.JCI insight · 2021Article
- Cell Atlas technologies and insights into tissue architecture.The Biochemical journal · 2020Review
- Imaging of Endoplasmic Reticulum CaFrontiers in endocrinology · 2020Article
- scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation.Genome biology · 2019Article
- Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Follicle-Stimulating Hormone Glycobiology.Endocrinology · 2019Review
- Cytogenetic, Genomic, and Functional Characterization of Pituitary Gonadotrope Cell Lines.Journal of the Endocrine Society · 2019Article
- Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.Frontiers in endocrinology · 2019Article
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
Immediate-early response genes (IEGs) are rapidly and transiently induced following an extracellular signal. Elucidating the IEG response patterns in single cells (SCs) requires assaying large numbers of timed samples at high accuracy while minimizing handling effects. To achieve this, we developed and validated RNA stabilization Buffer for Examination of Single-cell Transcriptomes (RNA-Best), a versatile single-step cell and tissue preservation protocol that stabilizes RNA in intact SCs without perturbing transcription patterns. We characterize for the first time SC heterogeneity in IEG responses to pulsatile gonadotropin-releasing hormone (GnRH) stimuli in pituitary gonadotrope cells. Our study identifies a gene-specific hierarchical pattern of all-or-none transcript induction elicited by increasing concentrations of GnRH. This quantal pattern of gene activation raises the possibility that IEG activation, when accurately resolved at the SC level, may be mediated by gene bits that behave as pure binary switches.
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Registered trials
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