ArticlePeerJ2018
Pushing the limits of whole genome amplification: successful sequencing of RADseq library from a single microhymenopteran (Chalcidoidea,
Article in PeerJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Evidence for balancing selection on loci associated with host use in the genome of a generalist plant parasite.Evolution letters · 2026Article
- Hookworm genomic diversity and population structure from accessible sample types: A validated approach to generate genome-wide polymorphism datasets from individual third-stage larvae.bioRxiv : the preprint server for biology · 2026Article
- Taxonomy and Biological Control: New Challenges in an Old Relationship.Neotropical entomology · 2023Article
- Cost-saving population genomic investigation ofEcology and evolution · 2022Article
- Evolution, systematics and historical biogeography of sand flies of the subgenus Paraphlebotomus (Diptera, Psychodidae, Phlebotomus) inferred using restriction-site associated DNA markers.PLoS neglected tropical diseases · 2021Article
- Disjunction between canola distribution and the genetic structure of its recently described pest, the canola flower midge (Ecology and evolution · 2020Article
- Hybrid Genome Assembly and Evidence-Based Annotation of the Egg Parasitoid and Biological Control AgentG3 (Bethesda, Md.) · 2020Article
- Article
- Genome-wide SNP Data Reveal an Overestimation of Species Diversity in a Group of Hawkmoths.Genome biology and evolution · 2019Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major obstacle to high-throughput genotyping of microhymenoptera is their small size. As species are difficult to discriminate, and because complexes may exist, the sequencing of a pool of specimens is hazardous. Thus, one should be able to sequence pangenomic markers (e.g., RADtags) from a single specimen. To date, whole genome amplification (WGA) prior to library construction is still a necessity as at most 10 ng of DNA can be obtained from single specimens (sometimes less). However, this amount of DNA is not compatible with manufacturer's requirements for commercial kits. Here we test the accuracy of the GenomiPhi kit V2 on
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.