ArticleJournal of virology2019
Isolation of Neutralizing Monoclonal Antibodies to Human Astrovirus and Characterization of Virus Variants That Escape Neutralization.
Article in Journal of virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Experimental Porcine Astrovirus 4-Associated Tracheitis and Bronchitis in Pigs.Transboundary and emerging diseases · 2026Article
- Structure of the human astrovirus capsid spike in complex with the neonatal Fc receptor.Nature communications · 2025Article
- Comparison of classic and novel human astrovirus MLB and VA seroprevalence in HIV and non-HIV cohorts in South China demonstrates high seroreactivity to classic human astrovirus, which is associated with HIV infection.Microbiology spectrum · 2025Article
- Functional characterization of DPP4 and FcRn as receptor and coreceptor for classical human astroviruses in Caco-2 cells.PLoS pathogens · 2025Article
- Identification of a novel linear B-cell epitope recognized by a monoclonal antibody against the capsid protein of goose astrovirus genotypes 2.BMC veterinary research · 2025Article
- Computational design of a glycosylated multi-epitope vaccine against HAsV-1 and HAsV-2 astrovirus for acute gastroenteritis.Scientific reports · 2025Article
- Isolation and characterization of goose astrovirus genotype 1 causing enteritis in goslings from Sichuan Province, China.BMC veterinary research · 2025Article
- Discovery of three novel neutralizing antibody epitopes on the human astrovirus capsid spike and mechanistic insights into virus neutralization.Journal of virology · 2025Article
- Article
- Discovery of three novel neutralizing antibody epitopes on the human astrovirus capsid spike and mechanistic insights into virus neutralization.bioRxiv : the preprint server for biology · 2024Article
- Mouse and human immune responses share neutralization epitopes of HAstV-VA1.Journal of virology · 2024Article
- Structure and antigenicity of the divergent human astrovirus VA1 capsid spike.PLoS pathogens · 2024Article
- Structure and immunogenicity of the murine astrovirus capsid spike.The Journal of general virology · 2023Article
- Human astrovirus capsid protein releases a membrane lytic peptide upon trypsin maturation.Journal of virology · 2023Article
- Establishment and Application of Indirect ELISAs for Detecting Antibodies against Goose Astrovirus Genotype 1 and 2.Vaccines · 2023Article
- Identification of a Novel Astrovirus Associated with Bovine Respiratory Disease.Transboundary and emerging diseases · 2023Article
- Structure of the divergent human astrovirus MLB capsid spike.Structure (London, England : 1993) · 2022Article
- Goose Astrovirus in China: A Comprehensive Review.Viruses · 2022Review
- The Capsid Precursor Protein of Astrovirus VA1 Is Proteolytically Processed Intracellularly.Journal of virology · 2022Article
- Bovine Astrovirus-A Comprehensive Review.Viruses · 2022Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Human astroviruses (HAstVs) cause severe diarrhea and represent an important health problem in children under two years of age. Despite their medical importance, the study of these pathogens has been neglected. To better understand the astrovirus antigenic structure and the basis of protective immunity, in this work we produced a panel of neutralizing monoclonal antibodies (Nt-MAbs) to HAstV serotypes 1, 2, and 8 and identified the mutations that allow the viruses to escape neutralization. We first tested the capacity of the recombinant HAstV capsid core and spike domains to elicit Nt-Abs. Hyperimmunization of animals with the two domains showed that although both induced a potent immune response, only the spike was able to elicit antibodies with neutralizing activity. Based on this finding, we used a mixture of the recombinant spike domains belonging to the three HAstV serotypes to immunize mice. Five Nt-MAbs were isolated and characterized; all of them were serotype specific, two were directed to HAstV-1, one was directed to HAstV-2, and two were directed to HAstV-8. These antibodies were used to select single and double neutralization escape variant viruses, and determination of the amino acid changes that allow the viruses to escape neutralization permitted us to define the existence of four potentially independent neutralization epitopes on the HAstV capsid. These studies provide the basis for development of subunit vaccines that induce neutralizing antibodies and tools to explore the possibility of developing a specific antibody therapy for astrovirus disease. Our results also establish a platform to advance our knowledge on HAstV cell binding and entry.
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