Evidence map›Paper›PMID 30355300›Full record

ArticleRadiation oncology (London, England)2018

Overexpression of HOTAIR leads to radioresistance of human cervical cancer via promoting HIF-1α expression.

Ning Li, Dan-Dan Meng, Ling Gao, Yue Xu, Pei-Jie Liu, Yong-Wei Tian, Zhen-Ying Yi, Yan Zhang, Xiao-Jing Tie, Zhi-Qiao Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Radiation oncology (London, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 62 citations in OpenAlex.

  1. Review
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  5. Article
  6. Review
  7. Promising predictive molecular biomarkers for cervical cancer (Review).International journal of molecular medicine · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Ning LiTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Dan-Dan MengTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Ling GaoTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Yue XuTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Pei-Jie LiuTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Yong-Wei TianTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Zhen-Ying YiTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Yan ZhangTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Xiao-Jing TieTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China.
Zhi-Qiao XuTumor Diagnosis and Treatment Center of Kaifeng Central Hospital, No 85 HeDao street, Longting District, Kaifeng, 475001, Henan, China. esc7312@163.com.
Kaifeng University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHOTAIR was known to enhance radioresistance in several cancers. However, the function of HOTAIR on radioresistance involving the regulation of HIF-1α in cervical cancer has not been reported.

methodsBALB/c nude mice were injected subcutaneously with HeLa cells and irradiated by X-ray. The tumor volume was measured and the expression of HOTAIR in tumors was detected by quantitative real-time PCR. Western blot was performed to detect the protein level of HIF-1α. MTT (3-(4,5-Dimethylthiazol-2-yl) 22,5-diphenyltetrazolium bromide) assay and the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to examine the cell viability and cell apoptosis of HeLa cells and C33A cells exposed to radiation.

resultsRadiotherapy inhibited the tumor growth in mice bearing HeLa cells. Radiotherapy reduced the expression of HOTAIR and HIF-1α in tumor tissues and HeLa cells or C33A cells. HOTAIR overexpression abrogated the effect of radiation on the cell viability and cell apoptosis of HeLa and C33A cells. HOTAIR also upregulated the expression of HIF-1α in HeLa and C33A cell exposed to radiation. HIF-1α knockdown reversed increasing cell viability and reducing apoptosis of HeLa and C33A cell induced by HOTAIR overexpression. HOTAIR overexpression promoted tumor growth in mice bearing HeLa and exposed to radiation.

conclusionRadiotherapy might inhibit cervical cancer cell growth through HOTAIR/HIF-1α pathway.

Indexed as

Radiation ToleranceAnimalsApoptosisCell SurvivalFemaleGene Expression Regulation, NeoplasticHumansHypoxia-Inducible Factor 1, alpha SubunitMiceMice, Inbred BALB CMice, NudeRadiation DosageRNA, Long NoncodingUterine Cervical NeoplasmsHIF1A protein, humanHif1a protein, mouseHOTAIR long non-coding RNA, mouseHOTAIR long untranslated RNA, humanHypoxia-Inducible Factor 1, alpha SubunitRNA, Long NoncodingCell apoptosisCervical cancerHIF-1αHOTAIRRadiotherapy

Identifiers

PMID30355300
PMCPMC6201557
OpenAlexW2898412101

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.