ArticleBMC microbiology2018
Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation.
Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 28 citations in OpenAlex.
- Metagenomics reveals an interaction among rhizosphere microbial community, soil properties and active ingredients in a medicinal cropJournal of ginseng research · 2026Article
- Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructose.Microbial cell factories · 2026Article
- A systems-level insight into PHB-driven metabolic adaptation orchestrated by the PHB-binding transcriptional regulator AniA (PhaR).mSystems · 2025Article
- Overexpression of RuBisCO form I and II genes inApplied and environmental microbiology · 2024Article
- Pleiotropic Effects of PhaR Regulator inInternational journal of molecular sciences · 2024Article
- Biosynthesis of polyhydroxybutyrate byFrontiers in plant science · 2024Article
- Use of high throughput DNA analysis to characterize the nodule-associated bacterial community from four ages ofAIMS microbiology · 2024Article
- Independent Component Analysis Reveals the Transcriptional Regulatory Modules inInternational journal of molecular sciences · 2023Article
- Construction of a Rhodobacter sphaeroides Strain That Efficiently Produces Hydrogen Gas from Acetate without Poly(β-Hydroxybutyrate) Accumulation: Insight into the Role of PhaR in Acetate Metabolism.Applied and environmental microbiology · 2022Article
- An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis.Microbial biotechnology · 2022Review
- What Did We Learn From Current Progress in Heat Stress Tolerance in Plants? Can Microbes Be a Solution?Frontiers in plant science · 2022Review
- Bacterial Exopolysaccharides: Insight into Their Role in Plant Abiotic Stress Tolerance.Journal of microbiology and biotechnology · 2021Review
- The Effects of Plant-Associated Bacterial Exopolysaccharides on Plant Abiotic Stress Tolerance.Metabolites · 2021Review
- How Rhizobia Adapt to the Nodule Environment.Journal of bacteriology · 2021Article
- A bacterial cell factory converting glucose into scyllo-inositol, a therapeutic agent for Alzheimer's disease.Communications biology · 2020Article
- Designing and EngineeringFrontiers in microbiology · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBradyrhizobium diazoefficiens USDA110 nodulates soybeans for nitrogen fixation. It accumulates poly-3-hydroxybutyrate (PHB), which is of physiological importance as a carbon/energy source for survival during starvation, infection, and nitrogen fixation conditions. PHB accumulation is orchestrated by not only the enzymes for PHB synthesis but also PHB-binding phasin proteins (PhaPs) stabilizing the PHB granules. The transcription factor PhaR controls the phaP genes.
resultsInactivation of phaR led to decreases in PHB accumulation, less cell yield, increases in exopolysaccharide (EPS) production, some improvement in heat stress tolerance, and slightly better growth under microaerobic conditions. Changes in the transcriptome upon phaR inactivation were analyzed. PhaR appeared to be involved in the repression of various target genes, including some PHB-degrading enzymes and others involved in EPS production. Furthermore, in vitro gel shift analysis demonstrated that PhaR bound to the promoter regions of representative targets. For the phaP1 and phaP4 promoter regions, PhaR-binding sites were determined by DNase I footprinting, allowing us to deduce a consensus sequence for PhaR-binding as TGCRNYGCASMA (R: A or G, Y: C or T, S: C or G, M: A or C). We searched for additional genes associated with a PhaR-binding sequence and found that some genes involved in central carbon metabolism, such as pdhA for pyruvate dehydrogenase and pckA for phosphoenolpyruvate carboxykinase, may be regulated positively and directly by PhaR.
conclusionsThese results suggest that PhaR could regulate various genes not only negatively but also positively to coordinate metabolism holistically in response to PHB accumulation.
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