ArticleBiochemistry2019
Two ABC Transporters and a Periplasmic Metallochaperone Participate in Zinc Acquisition in Paracoccus denitrificans.
Article in Biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Deep learning-drivenSynthetic and systems biotechnology · 2027Article
- Article
- A periplasmic zinc capture protein enhances the resistance ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Two zinc ABC transporters contribute toFrontiers in plant science · 2025Article
- Comprehensive Genomic Characterization ofMicroorganisms · 2023Article
- Exposure ofJournal of fungi (Basel, Switzerland) · 2023Article
- Zinc acquisition and its contribution toFrontiers in cellular and infection microbiology · 2023Article
- Structure and Function of the Zinc Binding Protein ZrgA fromInternational journal of molecular sciences · 2022Article
- Conformational flexibility in the zinc solute-binding protein ZnuA.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Contributions of Conformational Flexibility to High-Affinity Zinc Binding in the Solute Binding Protein AztC.ACS omega · 2022Article
- Zinc binding alters the conformational dynamics and drives the transport cycle of the cation diffusion facilitator YiiP.The Journal of general physiology · 2021Article
- An Insect- and Rain-Proof Net Raises the Production and Quality of Chinese Bayberry by Preventing Damage From Insects and Altering Bacterial Communities.Frontiers in plant science · 2021Article
- How Zinc-Binding Systems, Expressed by Human Pathogens, Acquire Zinc from the Colonized Host Environment: A Critical Review on Zincophores.Current medicinal chemistry · 2021Review
- Specificity of Interactions between Components of Two Zinc ABC Transporters inInternational journal of molecular sciences · 2020Article
- Metal Complexes of Two Specific Regions of ZnuA, a Periplasmic Zinc(II) Transporter fromInorganic chemistry · 2020Article
- Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins.Communications biology · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Bacteria must acquire the essential element zinc from extremely limited environments, and this function is performed largely by ATP binding cassette (ABC) transporters. These systems rely on a periplasmic or extracellular solute binding protein (SBP) to bind zinc specifically with a high affinity and deliver it to the membrane permease for import into the cytoplasm. However, zinc acquisition systems in bacteria may be more complex, involving multiple transporters and other periplasmic or extracellular zinc binding proteins. Here we describe the zinc acquisition functions of two zinc SBPs (ZnuA and AztC) and a novel periplasmic metallochaperone (AztD) in Paracoccus denitrificans. ZnuA was characterized in vitro and demonstrated to bind as many as 5 zinc ions with a high affinity. It does not interact with AztD, in contrast to what has been demonstrated for AztC, which is able to acquire a single zinc ion through associative transfer from AztD. Deletions of the corresponding genes singly and in combination show that either AztC or ZnuA is sufficient and essential for robust growth in zinc-limited media. Although AztD cannot support transport of zinc into the cytoplasm, it likely functions to store zinc in the periplasm for transfer through the AztABCD system.
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