ArticleJournal of cellular and molecular medicine2019
Rapid detection of low-level HeLa cell contamination in cell culture using nested PCR.
Article in Journal of cellular and molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- A decade-long retrospective analysis of cell line authentication in China using over 10,000 samples.Communications biology · 2026Article
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- Analysis of the effectiveness of improving the testing rate of pathogenicity specimens prior to the use of antibiotics in Shandong Province, 2014-2023.Frontiers in public health · 2025Article
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- The alpha-1A adrenergic receptor regulates mitochondrial oxidative metabolism in the mouse heart.Journal of molecular and cellular cardiology · 2024Article
- Review
- Single-molecule RNA in situ detection in clinical FFPE tissue sections by vsmCISH.RNA (New York, N.Y.) · 2023Article
- Vertically ordered mesoporous silica film-assisted electrochemical cytosensor for the sensitive detection of HeLa cells.Frontiers in chemistry · 2023Article
- Mutant SOD1 aggregates formed in vitro and in cultured cells are polymorphic and differ from those arising in the CNS.Journal of neurochemistry · 2023Article
- Clinicians' Intention to Submit Microbiological Pathogenic Test Before Antibiotics Use and Its Influencing Factors: New Evidence from the Perspective of Hospital Management.Infection and drug resistance · 2022Article
- Study of oral microorganisms contributing to non-carious cervical lesions via bacterial interaction and pH regulation.Journal of cellular and molecular medicine · 2021Article
- Article
- Rapid detection of low-level HeLa cell contamination in cell culture using nested PCR.Journal of cellular and molecular medicine · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HeLa cells are a commonly used cell line in many biological research areas. They are not picky for culture medium and proliferate rapidly. HeLa cells are a notorious source of cell cross-contamination and have been found to be able to contaminate a wide range of cell lines in cell culture. In this study, we reported a simple and efficient method for detecting the presence of HeLa cell contamination in cell culture. HPV-18 was used as a biomarker. The cell culture supernatant was used directly as the template for nested PCR without extracting nucleic acid. By PCR amplification of the cell culture supernatant with the designed primers, we were able to detect the presence of HeLa cells in the culture. The sensitivity of this method can reach 1%, which is 10-fold higher than Short tandem repeat sequence (STR) profiling. This simple, rapid, and "noninvasive" quality checking method should find applications in routine cell culture practice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.