ArticleNanoscale research letters2018
Red Spectral Shift in Sensitive Colorimetric Detection of Tuberculosis by ESAT-6 Antigen-Antibody Complex: a New Strategy with Gold Nanoparticle.
Article in Nanoscale research letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.
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Who cites it
11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Nanoparticles as an Alternative Strategy for the Rapid Detection ofIET nanobiotechnology · 2025Pooled it
- Current progress of functional nanobiosensors for potential tuberculosis diagnosis: The novel way for TB control?Frontiers in bioengineering and biotechnology · 2022Pooled it
- Antibody-aptamer Complementation: Advancing Biosensing for Disease Monitoring.Current topics in medicinal chemistry · 2026Review
- Mycobacteriophage Functionalized Magnetic Nanocrystal Clusters for Highly Sensitive and Rapid Detection ofJACS Au · 2025Article
- Review
- Recent advances in airborne pathogen detection using optical and electrochemical biosensors.Analytica chimica acta · 2022Review
- Non-protein coding RNA sequences mediate specific colorimetric detection of Staphylococcus aureus on unmodified gold nanoparticles.Scientific reports · 2022Article
- Current-Volt Biosensing "Cystatin C" on Carbon Nanowired Interdigitated Electrode Surface: A Clinical Marker Analysis for Bulged Aorta.Journal of analytical methods in chemistry · 2022Article
- Targeted DNA complementation on a 1,1'-carbonyldiimidazole-functionalized surface for identifying3 Biotech · 2020Article
- Coordination of Nanoconjugation with an Antigen/Antibody for Efficient Detection of Gynecological Tumors.Journal of analytical methods in chemistry · 2020Article
- Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis.Journal of analytical methods in chemistry · 2019Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuberculosis (TB) is a highly contagious life-threatening disease caused by the bacterial pathogen Mycobacterium tuberculosis. ESAT-6, an abundant early secretory antigenic target protein by M. tuberculosis, found to play a vital role in virulence. Developing a friendly method for the detection of ESAT-6 at the lower concentration facilitates to treat TB at an earlier stage and helps to control the spreading of disease. Herein, a new single-step approach was designed and was done by pre-mixing ESAT-6 and antibody before being added to the gold nanoparticle (GNP) followed by the salt-induced aggregation. We could attain the detection limit of 1.25 pM, showing the aggregation of GNP and the red spectral shift. Further, a higher specificity was demonstrated with the lack of electrostatic biofouling by ESAT-6 on GNP and retained the dispersed GNP in the presence of 10-kDa culture filtrate protein from M. tuberculosis. The required precise antibody concentration for this assay was found to be 60 nM. The increment in the antibody concentration from 75 nM drastically diminishes the sensitivity to ~ 680-fold, due to the crowding effect. With this assay, we attested the suitability of colorimetric assay for efficiently detecting the smaller-sized protein.
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