ArticleScientific reports2018
A highly stable laccase obtained by swapping the second cupredoxin domain.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- MutexaGPT: an intuition-to-design translator for physics-based enzyme engineering.Nature computational science · 2026Article
- Decoding the distribution, structure-function-redox potential relationship and recent advances in fungal laccases: a systematic approach.Bioprocess and biosystems engineering · 2026Review
- MutexaGPT: An Intuition-to-Design Translator for Physics-based Enzyme Engineering.Research square · 2026Article
- Improving the Properties of Laccase Through Heterologous Expression and Protein Engineering.Microorganisms · 2025Review
- Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22.Bioresources and bioprocessing · 2025Article
- Recent Progress and Future Prospects of Laccase Immobilization on MOF Supports for Industrial Applications.Applied biochemistry and biotechnology · 2024Review
- Fungal Laccases: Fundamentals, Engineering and Classification Update.Biomolecules · 2023Review
- Structural Insight into the Amino Acid Environment of the Two-Domain Laccase's Trinuclear Copper Cluster.International journal of molecular sciences · 2023Article
- Enzyme-Catalyzed Polymerization of Kraft Lignin fromPolymers · 2023Article
- Designed High-Redox Potential Laccases Exhibit High Functional Diversity.ACS catalysis · 2022Article
- Microaerobic conditions enhance laccase production from Rheinheimera sp. in an economical medium.Archives of microbiology · 2022Article
- Heterologous Expression, Engineering and Characterization of a Novel Laccase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis.Biotechnology for biofuels · 2021Article
- A Novel Enzyme-Based SPR Strategy for Detection of the Antimicrobial Agent Chlorophene.Biosensors · 2021Article
- Protein Engineering Approaches to Enhance Fungal Laccase Production inInternational journal of molecular sciences · 2021Article
- Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.Applied and environmental microbiology · 2020Article
- Laccases: structure, function, and potential application in water bioremediation.Microbial cell factories · 2019Review
- Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database.Applied microbiology and biotechnology · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The robustness of a high-redox potential laccase has been enhanced by swapping its second cupredoxin domain with that from another fungal laccase, which introduced a pool of neutral mutations in the protein sequence without affecting enzyme functionality. The new laccase showed outstanding stability to temperature, pH (2-9) and to organic solvents, while maintaining the ability to oxidize high-redox potential substrates. By engineering the signal peptide, enzyme secretion levels in Saccharomyces cerevisiae were increased, which allowed to purify the engineered enzyme for further characterization. The purified domain-swap laccase presented higher activity in the presence of ethanol or methanol, superior half-lives at 50-70 °C, improved stability at acidic pH, and similar catalytic efficiency for DMP albeit a lower one for ABTS (due to a shift in optimum pH). A new N-glycosylation site and a putative new surface salt-bridge were evaluated as possible determinants for the improved stability by site-directed mutagenesis. Although neither seemed to be strictly responsible for the improved thermostability, the new salt bridge was found to notably contribute to the high stability of the swapped enzyme in a broad pH range. Finally, the application potential of the new laccase was demonstrated with the enzymatic treatment of kraft lignin, an industrially relevant lignin stream, at high temperature, neutral pH and short incubation times.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.