Evidence map›Paper›PMID 30353099›Full record

ArticleScientific reports2018

Characterization and improved properties of Glutamine synthetase from Providencia vermicola by site-directed mutagenesis.

Wu Zuo, Leitong Nie, Ram Baskaran, Ashok Kumar, Ziduo Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. First Genome Description ofMicroorganisms · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Wu ZuoCollege of Life Science and Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
Leitong NieCollege of Life Science and Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
Ram BaskaranCollege of Life Science and Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
Ashok KumarDepartment of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, 173234, Himachal Pradesh, India.
Ziduo LiuCollege of Food and Bio-science and Technology, Wuhan Institute of Design and Science, Wuhan, 430205, P.R. China. lzd@mail.hzau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, a novel gene for Glutamine synthetase was cloned and characterized for its activities and stabilities from a marine bacterium Providencia vermicola (PveGS). A mutant S54A was generated by site directed mutagenesis, which showed significant increase in the activity and stabilities at a wide range of temperatures. The K

Indexed as

Mutagenesis, Site-DirectedAmmonium CompoundsBinding SitesCloning, MolecularDetergentsEnzyme StabilityGlutamate-Ammonia LigaseHydrogen-Ion ConcentrationIndicators and ReagentsKineticsLigandsMetalsMolecular Docking SimulationMutant ProteinsProvidenciaStructural Homology, ProteinAmmonium CompoundsDetergentsGlutamate-Ammonia LigaseIndicators and ReagentsLigandsMetalsMutant Proteins

Identifiers

PMID30353099
PMCPMC6199252

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.