Evidence map›Paper›PMID 30352968›Full record

ArticleCells2018

Telomere Length Calibration from qPCR Measurement: Limitations of Current Method.

Youjin Wang, Sharon A Savage, Rotana Alsaggaf, Geraldine Aubert, Casey L Dagnall, Stephen R Spellman, Stephanie J Lee, Belynda Hicks, Kristine Jones, Hormuzd A Katki and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Youjin WangClinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. youjin.wang@nih.gov.
Sharon A SavageClinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. savagesh@mail.nih.gov.
Rotana AlsaggafClinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. rotana.alsaggaf@nih.gov.
Geraldine AubertTerry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada. gaubert@bccrc.ca.
Casey L DagnallCancer Genomics Research Laboratory, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. dagnallc@mail.nih.gov.ORCID 0000-0001-7334-4718
Stephen R SpellmanCenter for International Blood and Marrow Transplant Research, Minneapolis, MN 55401, USA. sspellma@nmdp.org.
Stephanie J LeeCenter for International Blood and Marrow Transplant Research, Medical College of Wisconsin, Milwaukee, WI 53226, USA. sjlee@fredhutch.org.
Belynda HicksCancer Genomics Research Laboratory, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. hicksbel@mail.nih.gov.ORCID 0000-0001-8014-4888
Kristine JonesCancer Genomics Research Laboratory, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. kristine.jones@nih.gov.
Hormuzd A KatkiBiostatistics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. katkih@mail.nih.gov.
Shahinaz M GadallaClinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. shahinaz.gadalla@nih.gov.

Funding

Data Resource for Analyzing Blood &Marrow TransplantsU24CA076518 · NCI · MEDICAL COLLEGE OF WISCONSIN · PI Amy M Moskop, Bronwen Shaw · 1998 to 2026
$105.2M
Genetic Epidemiology of Telomere Maintenance and Cancer EtiologyZIACP010190 · NCI · DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS · PI SAVAGE, SHARON A. · 2009 to 2025
$10.2M
PROLACTIN RECEPTOR COMPLEX IN BREAST CANCERR01CA069294 · NCI · UNIVERSITY OF PENNSYLVANIA · PI CLEVENGER, CHARLES V · 1996 to 2006
$1.9M
Health Resources and Services Administration (HRSA/DHHS) HHSH250201200018CNational Cancer Institute, National Heart, Lung and Blood Institute and National Institute of Allergy and Infectious Diseases 5U24CA076518National Cancer Institute, National Institutes of Health The Intramural Research Program of the Division of Cancer Epidemiology and GeneticsNational Heart, Lung and Blood Institute and National Cancer Institute 4U10HL069294The Office of Naval Research N00014-17-1-2388The Office of Naval Research N0014-17-1-2850
6 · The paper itself

Abstract

Telomere length (TL) comparisons from different methods are challenging due to differences in laboratory techniques and data configuration. This study aimed to assess the validity of converting the quantitative polymerase chain reaction (qPCR) telomere/single copy gene (T/S) ratio to TL in kilobases (kb). We developed a linear regression equation to predict TL from qPCR T/S using flow cytometry with fluorescence in situ hybridization (flow FISH) TL data from 181 healthy donors (age range = 19⁻53) from the National Marrow Donor Program (NMDP) biorepository. TL measurements by qPCR and flow FISH were modestly correlated (

Indexed as

agreementflow FISHqPCRtelomere length

Identifiers

PMID30352968
PMCPMC6262465

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.