ArticleMedical principles and practice : international journal of the Kuwait University, Health Science Centre2019
Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus.
Article in Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
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- The performance of hybrid materials for the biodegradation of dichloromethane using Pseudomonas aeruginosa, coconut shell, rice husk, and metal organic framework.Biodegradation · 2025Article
- Antimicrobial Resistance Profile and Biofilm Formation ofAntibiotics (Basel, Switzerland) · 2025Article
- Pathogen Partnerships or Power Struggles? Pseudomonas aeruginosa, and Staphylococcus aureus Dynamics in Cystic Fibrosis.Current microbiology · 2025Article
- Influence of Amino Acids on Quorum Sensing-Related Pathways inMetabolites · 2025Article
- Carbon-Infiltrated Carbon Nanotube Topography Reduces the Growth ofNanomaterials (Basel, Switzerland) · 2025Article
- A screening setup to streamlineMaterials today. Bio · 2025Article
- Integrated genomic and proteomic analysis of the mouse-adaptedCurrent research in microbial sciences · 2025Article
- Histamine Promotes Pseudomonas aeruginosa Biofilm Formation and Renders P. aeruginosa Biofilms More Resistant to Gentamicin and Azithromycin.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2025Article
- Pathogen exclusion from intestinal mucus and antimicrobial susceptibility ofMicrobiome research reports · 2025Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivePseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. MATERIALS AND
methodsWe monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and scanning electron microscopy (SEM).
resultsThe most robust growth of the mono- and dual-species cultures was noted in BHI broth. On the contrary, RPMI 1640 medium promoted maximal initial adhesion of both the mono- and dual-species, but BHI broth fostered the maximal biofilm growth. SEM images showed profuse extracellular polysaccharide production in biofilms, particularly in coculture, in BHI medium.
conclusionOur data demonstrate that BHI broth, relative to the other tested media, is the most conducive for in vitro evaluation of biofilm and planktonic growth kinetics of these two pathogens, both in mono- and coculture.
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