ArticleThe journal of physical chemistry. B2018
Direct Observation of Tunneling Nanotubes within Human Mesenchymal Stem Cell Spheroids.
Article in The journal of physical chemistry. B, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Tunneling nanotubes: a new dimension of intercellular communication and recent progress.Cell communication and signaling : CCS · 2026Review
- Human neural stem cells derived from fetal human brain communicate with each other and rescue ischemic neuronal cells through tunneling nanotubes.Cell death & disease · 2024Article
- Cell-cell communication: new insights and clinical implications.Signal transduction and targeted therapy · 2024Review
- Fibril-Guided Three-Dimensional Assembly of Human Fibroblastic Reticular Cells.ACS applied bio materials · 2024Article
- Article
- Intercellular Highways in Transport Processes.Results and problems in cell differentiation · 2024Review
- Role of Tunneling Nanotubes in the Nervous System.International journal of molecular sciences · 2022Review
- Tunneling nanotubes and mesenchymal stem cells: New insights into the role of melatonin in neuronal recovery.Journal of pineal research · 2022Review
- 3D nanoprinting via spatially controlled assembly and polymerization.Nature communications · 2022Article
- Mitochondria: Insights into Crucial Features to Overcome Cancer Chemoresistance.International journal of molecular sciences · 2021Review
- Peering into tunneling nanotubes-The path forward.The EMBO journal · 2021Review
- Tunneling nanotubes mediate the expression of senescence markers in mesenchymal stem/stromal cell spheroids.Stem cells (Dayton, Ohio) · 2020Article
- Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging.BioMed research international · 2020Review
- Tunneling nanotubes: A bridge for heterogeneity in glioblastoma and a new therapeutic target?Cancer reports (Hoboken, N.J.) · 2019Review
- Intracrine Endorphinergic Systems in Modulation of Myocardial Differentiation.International journal of molecular sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Tunneling nanotubes (TNTs) play an important role in cell-cell communication. TNTs have been predominantly reported among cells in monolayer culture. Using various imaging modalities, including scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM), this work reports the finding of TNTs between cells within human mesenchymal stem cell (MSC) spheroids. TNTs visualized by SEM are consistent in size and geometry with those observed in cellular monolayer culture. LSCM imaging of living spheroids confirms the presence of F-actin filaments within the TNTs, which are known to maintain nanotube integrity. In addition, LSCM revealed the distribution of F-actin fibers across the entire spheroid body instead of being confined within individual cells. Intracellular material transport by TNTs was tested in MSC spheroids treated with cytochalasin D (CytoD), a known actin polymerization inhibitor for disrupting TNT formation. CytoD treatment decreased the transport of cytosolic material by at least four-fold compared to untreated spheroids. To the best of our knowledge, this work represents the first direct observation of TNTs within MSC spheroids. These findings offer new physical insight into cellular interactions within spheroids, providing structural information for increasing interests in spheroid-based cell therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.