Evidence map›Paper›PMID 30350225›Full record

ArticleExtremophiles : life under extreme conditions2019

In silico and experimental improvement of bacteriorhodopsin production in Halobacterium salinarum R1 by increasing DNA-binding affinity of Bat through Q661R/Q665R substitutions in HTH motif.

Azadeh Mirfeizollahi, Bagher Yakhchali, Ali Asghar Deldar, Ali Asghar Karkhane

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Article in Extremophiles : life under extreme conditions, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Genes · 2019
    Article
4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Azadeh MirfeizollahiInstitute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Km 15, Tehran-Karaj Highway, P.O. Box 14965/161, Tehran, Iran.
Bagher YakhchaliInstitute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Km 15, Tehran-Karaj Highway, P.O. Box 14965/161, Tehran, Iran. bahar@nigeb.ac.ir.
Ali Asghar DeldarDepartment of Bioscience, Institute of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
Ali Asghar KarkhaneInstitute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Km 15, Tehran-Karaj Highway, P.O. Box 14965/161, Tehran, Iran.
National Institute of Genetic Engineering and Biotechnology · IRMalek Ashtar University of Technology · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA-binding motif of bacterioopsin activator (Bat) protein is a Helix-Turn-Helix motif, which binds to bop promoter and induces bacterioopsin (Bop) expression under light and low oxygen tension. Bacterioopsin is linked to retinal to produce bacteriorhodopsin (BR), which in turn supplies energy source in Halobacterium salinarum. In this study, effect of Bat HTH motif-promoter DNA interaction on bacterioopsin (Bop) expression was investigated using in silico and experimental approaches. Molecular docking showed that the most stable DNA-protein complex was generated by Q661R/Q665R mutant. Based on the in silico analysis, HTH motif was mutated using site-directed mutagenesis and Hbt. salinarum recombinant strains were developed by introduction of mutant bat genes. Double positively charged amino acid substitutions (Q661R/Q665R) in second helix of HTH motif increased whereas deletion of this region decreased BR production. However, other single substitutions (Q665R and Q661H) did not change BR production. These findings represent key role of HTH motif stability for DNA binding and regulation of bacterioopsin (Bop) expression and bacteriorhodopsin (BR) production independent of environmental condition.

Indexed as

BacteriorhodopsinsBinding SitesHalobacterium salinarumIndustrial MicrobiologyMolecular Docking SimulationMutation, MissensePromoter Regions, GeneticProtein BindingTranscription FactorsBacteriorhodopsinsTranscription FactorsAmino acid substitutionBacteriorhodopsinHalobacterium salinarumHTH motifMolecular dockingRegulatory protein

Identifiers

PMID30350225
OpenAlexW2897094410

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.