ArticleNature microbiology2018
A surface endogalactanase in Bacteroides thetaiotaomicron confers keystone status for arabinogalactan degradation.
Article in Nature microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 163 citations in OpenAlex.
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- Distribution of microbial carrageenan foraging pathways reveals a widespread latent trait within the ruminant intestinal microbiome.Nature communications · 2026Article
- Degradation of mucinbioRxiv : the preprint server for biology · 2026Article
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- Structural insights into lacto-N-biose I recognition by a family 32 carbohydrate-binding module from Bifidobacterium bifidum.FEBS letters · 2026Article
- Integrative multi-omics dissection identifies ACO2, KLF5, and IMP4 as central regulators of the mitochondrial-immune axis in ulcerative colitis.Frontiers in immunology · 2026Article
- Spatial proximity dictates bacterial competition and expansion in microbial communities.Nature communications · 2025Article
- Metabolic modeling of microbial communities in the chicken ceca reveals a landscape of competition and co-operation.Microbiome · 2025Article
- Transcriptional regulatory networks of the human gut symbiont Bacteroides thetaiotaomicron are uncovered using machine learning.Nucleic acids research · 2025Article
- β-galactanase: an effective tool for the degradation of plant β-ᴅ-galactan.World journal of microbiology & biotechnology · 2025Review
- Contiguous and complete assemblies ofGenome research · 2025Article
- Deceptive Ceropegia sandersonii uses an arabinogalactan for trapping its fly pollinators.The New phytologist · 2025Article
- Enzymatic oxidation of galacturonides from pectin breakdown contributes to stealth infection by Oomycota phytopathogens.Nature communications · 2025Article
- In vivo manipulation of human gut Bacteroides fitness by abiotic oligosaccharides.Nature chemical biology · 2025Article
- Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium.Communications biology · 2025Article
- Evaluating the prebiotic activity of arabinogalactan on the human gut microbiota using 16S rRNA gene sequencing and Raman-activated cell sorting.Microbiome research reports · 2025Article
- Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks.Journal of animal science and technology · 2024Article
- A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans.NPJ biofilms and microbiomes · 2024Article
- Dietary fiber monosaccharide content alters gut microbiome composition and fermentation.Applied and environmental microbiology · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
19 authors at 5 institutions in 4 countries.
Funding
Abstract
Glycans are major nutrients for the human gut microbiota (HGM). Arabinogalactan proteins (AGPs) comprise a heterogenous group of plant glycans in which a β1,3-galactan backbone and β1,6-galactan side chains are conserved. Diversity is provided by the variable nature of the sugars that decorate the galactans. The mechanisms by which nutritionally relevant AGPs are degraded in the HGM are poorly understood. Here we explore how the HGM organism Bacteroides thetaiotaomicron metabolizes AGPs. We propose a sequential degradative model in which exo-acting glycoside hydrolase (GH) family 43 β1,3-galactanases release the side chains. These oligosaccharide side chains are depolymerized by the synergistic action of exo-acting enzymes in which catalytic interactions are dependent on whether degradation is initiated by a lyase or GH. We identified two GHs that establish two previously undiscovered GH families. The crystal structures of the exo-β1,3-galactanases identified a key specificity determinant and departure from the canonical catalytic apparatus of GH43 enzymes. Growth studies of Bacteroidetes spp. on complex AGP revealed 3 keystone organisms that facilitated utilization of the glycan by 17 recipient bacteria, which included B. thetaiotaomicron. A surface endo-β1,3-galactanase, when engineered into B. thetaiotaomicron, enabled the bacterium to utilize complex AGPs and act as a keystone organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.