ArticleMicrobial cell factories2018
Identification of sesquiterpene synthases from the Basidiomycota Coniophora puteana for the efficient and highly selective β-copaene and cubebol production in E. coli.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Characterization of the Cubamyces Menziesii Terpenome.Chembiochem : a European journal of chemical biology · 2025Article
- Genomic and Multi-Omics Analysis ofJournal of fungi (Basel, Switzerland) · 2025Article
- Two Terpene Synthases Are Involved in Multiple Sesquiterpene Biosynthesis in the Woody Vegetable,International journal of molecular sciences · 2025Article
- Hierarchical metabolic engineering for rewiring cellular metabolism.FEMS microbiology reviews · 2025Review
- Genomic Features ofJournal of fungi (Basel, Switzerland) · 2024Article
- Genome Mining of Fungal Unique Trichodiene Synthase-like Sesquiterpene Synthases.Journal of fungi (Basel, Switzerland) · 2024Article
- Antibacterial Efficiency ofAntibiotics (Basel, Switzerland) · 2023Article
- Molecular and Functional Analyses of Characterized Sesquiterpene Synthases in Mushroom-Forming Fungi.Journal of fungi (Basel, Switzerland) · 2023Article
- Impact of Agro-Industrial Side-Streams on Sesquiterpene Production by Submerged CulturedFoods (Basel, Switzerland) · 2023Article
- Biosynthesis of Sesquiterpenes in Basidiomycetes: A Review.Journal of fungi (Basel, Switzerland) · 2022Review
- Ectomycorrhizal Influence on the Dynamics of Sesquiterpene Release byJournal of fungi (Basel, Switzerland) · 2022Article
- Phytochemical Compositions and Antioxidant Activities of Essential Oils Extracted from the Flowers ofMolecules (Basel, Switzerland) · 2022Article
- Biotechnological potential and initial characterization of two novel sesquiterpene synthases from Basidiomycota Coniophora puteana for heterologous production of δ-cadinol.Microbial cell factories · 2022Article
- De novo biosynthesis of τ-cadinol in engineered Escherichia coli.Bioresources and bioprocessing · 2022Article
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
- Biosynthesis and regulation of terpenoids from basidiomycetes: exploration of new research.AMB Express · 2021Review
- Genome reduction and relaxed selection is associated with the transition to symbiosis in the basidiomycete genusiScience · 2021Article
- Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research.Biology open · 2020Review
- The foxtail millet (Setaria italica) terpene synthase gene family.The Plant journal : for cell and molecular biology · 2020Article
- Exploring the catalytic cascade of cembranoid biosynthesis by combination of genetic engineering and molecular simulations.Computational and structural biotechnology journal · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundTerpenes are an important and extremely versatile class of secondary metabolites that are commercially used in the pharmaceutical, food and cosmetics sectors. Genome mining of different fungal collections has revealed the genetic basis for a steadily increasing number of putative terpene synthases without any detailed knowledge about their biochemical properties. The analysis and research of this rich genetic source provides a precious basis for the advancing biotechnological production of an almost endless number of valuable natural metabolites.
resultsThree annotated terpene synthases from the little investigated Basidiomycota Coniophora puteana were studied in this work. For biochemical characterization, the heterologous expression in E. coli was conducted leading to the identification of two sesquiterpene synthases capable of the highly selective generation of β-copaene and cubebol. These compounds are commercially used as food and flavor additives. The new enzymes show the highest reported product selectivity for their main compounds and therefore represent the first exclusive synthases for β-copaene (62% product selectivity) and cubebol (75% product selectivity) generation. In combination with an optimized heterologous microbial production system, we obtained product titers of 215 mg/L β-copaene and 497 mg/L cubebol.
conclusionThe reported product selectivity and our generated terpene titers exceed all published biotechnological data regarding the production of β-copaene and cubebol. This represents a promising and economic alternative to extraction from natural plant sources and the associated complex product purification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.