ArticleInternational journal of molecular sciences2018
How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Microbiota profiles in pre-school children with respiratory infections: Modifications induced by the oral bacterial lysate OM-85.Frontiers in cellular and infection microbiology · 2022Trial
- DNA sequencing for microbial surveillance in cystic fibrosis airways: advances, challenges, and clinical translation.Clinical microbiology reviews · 2026Review
- Differential Metabolite Production Underlies Disruption of the Cystic Fibrosis Airway Microbiota by Pathogens.bioRxiv : the preprint server for biology · 2026Article
- Application and concentration selection of dithiothreitol for correcting false elevation of D-dimer.Practical laboratory medicine · 2025Article
- Comparative evaluation of proteinase K and dithiothreitol as pretreatments for extracting nucleic acids from respiratory samples for multiplex PCR.BMC microbiology · 2025Article
- Detecting Respiratory Pathogens for Diagnosing Lower Respiratory Tract Infections at the Point of Care: Challenges and Opportunities.Biosensors · 2025Review
- Physiology and pathophysiology of mucus and mucolytic use in critically ill patients.Critical care (London, England) · 2025Review
- Metrological evaluation of DNA extraction method effects on the bacterial microbiome and resistome in sputum.mSystems · 2024Article
- Pro-resolving and pro-inflammatory fatty acid-derived mediators in sputum of stable state bronchiectasis patients.Respiratory research · 2022Observational
- Exome and Sputum Microbiota as Predictive Markers of Frequent Exacerbations in Chronic Obstructive Pulmonary Disease.Biomolecules · 2022Article
- Genome Sequence of a Potent Biosurfactant-Producing Bacterium,Microbiology resource announcements · 2022Article
- Rapid expansion and extinction of antibiotic resistance mutations during treatment of acute bacterial respiratory infections.Nature communications · 2022Article
- Metagenomic Assessment of the Pathogenic Risk of Microorganisms in Sputum of Postoperative Patients With Pulmonary Infection.Frontiers in cellular and infection microbiology · 2022Article
- Revisiting the controversy: The role of fungi in chronic rhinosinusitis.International forum of allergy & rhinology · 2021Review
- Lung microbiome of stable and exacerbated COPD patients in Tshwane, South Africa.Scientific reports · 2021Article
- Comparison of targeted metagenomics and IS-Pro methods for analysing the lung microbiome.BMC microbiology · 2021Article
- High-Throughput Metagenomics for Identification of Pathogens in the Clinical Settings.Small methods · 2021Review
- Commentary: Dithiothreitol (DTT), When Used as Biofilm Detaching Method to Diagnose Implant-Associated Infections, Does Not Affect Microorganisms' Viability, According to the Current Literature.Frontiers in microbiology · 2021Article
- Assessment of two DNA extraction kits for profiling poultry respiratory microbiota from multiple sample types.PloS one · 2021Article
- Impact of DNA Extraction Method on Variation in Human and Built Environment Microbial Community and Functional Profiles Assessed by Shotgun Metagenomics Sequencing.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of sputum. Sputum treatments with and without DTT were compared in terms of their median levels and the coefficient of variation between replicates of both DNA extraction yield and real-time PCR for the 16S rRNA gene. Treatments with and without lysozyme and lysostaphin were compared in terms of their median levels of real-time PCR for
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.