Evidence map›Paper›PMID 30347468›Full record

ArticleJournal of mass spectrometry : JMS2019

Top-down analysis of novel synthetic branched proteins.

Fabio Gomes, Betsegaw Lemma, Dulith Abeykoon, Dapeng Chen, Yan Wang, David Fushman, Catherine Fenselau

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of mass spectrometry : JMS, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Fabio GomesDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.ORCID http://orcid.org/0000-0003-0542-6820
Betsegaw LemmaDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.
Dulith AbeykoonDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.
Dapeng ChenDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.ORCID http://orcid.org/0000-0001-8412-581X
Yan WangProteomic Core Facility, University of Maryland, College Park, MD, 20742, USA.ORCID http://orcid.org/0000-0003-1187-9527
David FushmanDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.ORCID http://orcid.org/0000-0002-6634-8056
Catherine FenselauDepartment of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA.ORCID http://orcid.org/0000-0003-4979-0229

Funding

Ubiquitinated Proteins in Exosomes of Immune Suppressive Myeloid CellsR01GM021248 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI FENSELAU, CATHERINE C, FUSHMAN, DAVID · 1985 to 2017
$4.6M
Orbitrap Fusion Tribrid Mass SpectrometerS10OD019938 · OD · UNIV OF MARYLAND, COLLEGE PARK · PI FENSELAU, CATHERINE C · 2015 to 2015
$600k
APPLICATIONS OF MASS SPECTROMETRY IN PHARMACOLOGYR37GM021248 · NIGMS · UNIVERSITY OF MARYLAND COLLEGE PK CAMPUS · PI FENSELAU, CATHERINE C. · 1992 to 2001
$438k
National Science Foundation MCB1818280NIGMS NIH HHS R01 GM021248NIGMS NIH HHS R37 GM021248NIH HHS S10 OD019938US National Institutes of Health GM021248US National Institutes of Health OD019938
6 · The paper itself

Abstract

A strategy for top-down analysis of branched proteins has been reported earlier, which relies on electron transfer dissociation assisted by collisional activation, and software designed for graphic interpretation of tandem mass spectra and adapted for branched proteins. In the present study, the strategy is applied to identify unknown and novel products of reactions in which rationally mutated proteoforms of Rub1 are used to probe the selectivity of E1 and E2 enzymes normally active in ubiquitination. To test and demonstrate this application, components and attachment sites of three branched dimers are deduced and the mutations are confirmed.

Indexed as

UbiquitinChromatography, High Pressure LiquidElectron TransportTandem Mass SpectrometryUbiquitinationUbiquitinbranched proteinsgraphical softwareRub1rubylationtop-down mass spectrometry

Identifiers

PMID30347468
PMCPMC7236025

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.