ArticleSynthetic and systems biotechnology2018
Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions.
Article in Synthetic and systems biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 28 citations in OpenAlex.
- De Novo Biosynthesis of Valinomycin From Glucose Using In Vitro Reconstituted Hybrid Pathways.Chembiochem : a European journal of chemical biology · 2026Article
- Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem.Synthetic and systems biotechnology · 2023Article
- Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes.Advances in biochemical engineering/biotechnology · 2023Article
- Engineering of a thermophilic dihydroxy-acid dehydratase toward glycerate dehydration for in vitro biosystems.Applied microbiology and biotechnology · 2022Article
- Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering inSynthetic and systems biotechnology · 2020Article
- Development of an Improved Peroxidase-Based High-Throughput Screening for the Optimization of D-Glycerate Dehydratase Activity.International journal of molecular sciences · 2020Article
- Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid.Frontiers in bioengineering and biotechnology · 2019Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
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Abstract
Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the production of desired products from low-cost substrates. Here, a cell-free synthetic biosystem containing minimized number of reactions was designed for the conversion of d-glucose to l-lactate via pyruvate. This NADH-balanced biosystem was comprised of only 5 thermophilic enzymes without ATP supplementation. After optimization of enzyme loading amounts, buffer concentration and cofactor concentration, d-glucose was converted to l-lactate with a product yield of ∼90%. Our study has provided an emerging platform with potentials in producing pyruvate-derived chemicals, and may promote the development of cell-free synthetic enzymatic biosystems for biomanufacturing.
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