ArticleWellcome open research2018
A versatile LC-MS/MS approach for comprehensive, quantitative analysis of central metabolic pathways.
Article in Wellcome open research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
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- Branched-chain α-keto acids impair glucose-stimulated insulin secretion in pancreatic β-cells under diabetes by reactivating the LDHA-lactate axis.Nature communications · 2026Article
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
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- Macrophage metabolic reprogramming during dietary stress influences adult body size in Drosophila.EMBO reports · 2025Article
- Bioenergetic reprogramming of macrophages reduces drug tolerance in Mycobacterium tuberculosis.Nature communications · 2025Article
- Metabolic coupling of ROS generation and antioxidant synthesis by the GABA shunt pathway in myeloid-like blood progenitor cells of Drosophila.PLoS genetics · 2025Article
- Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathways.bioRxiv : the preprint server for biology · 2025Article
- A conserved editing mechanism for the fidelity of bacterial cell wall biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Isolation of Mitochondria From Yeast to Estimate Mitochondrial Pools of Inorganic Phosphate.Bio-protocol · 2025Article
- Active control of mitochondrial network morphology by metabolism-driven redox state.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Diurnal variation in skeletal muscle mitochondrial function dictates time-of-day-dependent exercise capacity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- The deubiquitinase Ubp3/Usp10 constrains glucose-mediated mitochondrial repression via phosphate budgeting.eLife · 2024Article
- Metabolic adaptation pilots the differentiation of human hematopoietic cells.Life science alliance · 2024Article
- Cold-shock proteome of myoblasts reveals role of RBM3 in promotion of mitochondrial metabolism and myoblast differentiation.Communications biology · 2024Article
- The PP2A-like phosphatase Ppg1 mediates assembly of the Far complex to balance gluconeogenic outputs and enables adaptation to glucose depletion.PLoS genetics · 2024Article
- Cysteine desulfurase (IscS)-mediated fine-tuning of bioenergetics and SUF expression preventsScience advances · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Liquid chromatography-mass spectrometry (LC-MS/MS) based approaches are widely used for the identification and quantitation of specific metabolites, and are a preferred approach towards analyzing cellular metabolism. Most methods developed come with specific requirements such as unique columns, ion-pairing reagents and pH conditions, and typically allow measurements in a specific pathway alone. Here, we present a single column-based set of methods for simultaneous coverage of multiple pathways, primarily focusing on central carbon, amino acid, and nucleotide metabolism. We further demonstrate the use of this method for quantitative, stable isotope-based metabolic flux experiments, expanding its use beyond steady-state level measurements of metabolites. The expected kinetics of label accumulation pertinent to the pathway under study are presented with some examples. The methods discussed here are broadly applicable, minimize the need for multiple chromatographic resolution methods, and highlight how simple labeling experiments can be valuable in facilitating a comprehensive understanding of the metabolic state of cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.