ArticleMolecular plant pathology2019
DCL-suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology.
Article in Molecular plant pathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Competition among co-infecting potyviral chimeras with adaptive, single-nucleotide differences in HCPro: Properties, and influence of environment factors on outcomes.PLoS pathogens · 2026Article
- Mixed viral infections uncover multifaceted RNA silencing strategies in plant antiviral defense.BMC genomics · 2026Article
- Transposon expansion is associated with reorganization of small RNA and DNA methylation landscapes in the morphologically minimal angiosperm Wolffia brasiliensis.The Plant journal : for cell and molecular biology · 2026Article
- Review
- HTS analysis of resistance induction against PPV by four hairpin constructs in Nicotiana benthamiana Domin.Scientific reports · 2025Article
- 3D-CNN detection of systemic symptoms induced by different Potexvirus infections in four Nicotiana benthamiana genotypes using leaf hyperspectral imaging.Plant methods · 2025Article
- Suppression of SMXL4 and SMXL5 confers enhanced thermotolerance through promoting HSFA2 transcription in Arabidopsis.The Plant cell · 2024Article
- Stress-related transcriptomic changes associated with GFP transgene expression and active transgene silencing in plants.Scientific reports · 2024Article
- Adaptive substitutions at two amino acids of HCPro modify its functional properties to separately increase the virulence of a potyviral chimera.Molecular plant pathology · 2024Article
- Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition.Nature communications · 2023Article
- Genome-Wide Identification and Characterization of Major RNAi Genes Highlighting Their Associated Factors in Cowpea (BioMed research international · 2023Article
- PSTVd infection inFrontiers in plant science · 2023Article
- Transcriptome analyses unveiled differential regulation of AGO and DCL genes by pepino mosaic virus strains.Molecular plant pathology · 2022Article
- Adaptation of a Potyvirus Chimera Increases Its Virulence in a Compatible Host through Changes in HCPro.Plants (Basel, Switzerland) · 2022Article
- The Carboxyl Terminal Regions of P0 Protein Are Required for Systemic Infections of Poleroviruses.International journal of molecular sciences · 2022Article
- Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genetic evidence for the involvement of Dicer-like 2 and 4 as well as Argonaute 2 in theThe Journal of general virology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing is a universal mechanism involved in development, epigenetic modifications and responses to biotic and abiotic stresses. The major components of this mechanism are Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins. Understanding the role of each component is of great scientific and agronomic importance. Plants, including Nicotiana benthamiana, an important plant model, usually possess four DCL proteins, each of which has a specific role, namely being responsible for the production of an exclusive small RNA population. Here, we used RNA interference (RNAi) technology to target DCL proteins and produced single and combinatorial mutants for DCL. We analysed the phenotype for each DCL knockdown plant, together with the small RNA profile, by next-generation sequencing (NGS). We also investigated transgene expression, as well as viral infections, and were able to show that DCL suppression results in distinct developmental defects, changes in small RNA populations, increases in transgene expression and, finally, higher susceptibility in certain RNA viruses. Therefore, these plants are excellent tools for the following: (i) to study the role of DCL enzymes; (ii) to overexpress proteins of interest; and (iii) to understand the complex relationship between the plant silencing mechanism and biotic or abiotic stresses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.