ArticleJournal of immunological methods2019
Immunofluorescence staining of live lymph node tissue slices.
Article in Journal of immunological methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Modeling Immunity In Vitro: Slices, Chips, and Engineered Tissues.Annual review of biomedical engineering · 2021Pooled it
- Functional profiling of porcine precision-cut lymph node slices as anFrontiers in immunology · 2026Article
- Ex Vivo Model of Breast Cancer Cell Invasion in Live Lymph Node Tissue.ACS pharmacology & translational science · 2025Article
- Initiation of primary T cell-B cell interactions and extrafollicular antibody responses in an organized microphysiological model of the human lymph node.bioRxiv : the preprint server for biology · 2025Article
- A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node.Lab on a chip · 2025Article
- Immune organoids: emerging platforms for modeling and analyzing human adaptive immunity.Frontiers in immunology · 2025Review
- Article
- Ex vivo model of breast cancer cell invasion in live lymph node tissue.bioRxiv : the preprint server for biology · 2024Article
- Spatially resolved quantification of oxygen consumption rate inThe Analyst · 2024Article
- B4 suppresses lymphoma progression by inhibiting fibroblast growth factor binding protein 1 through intrinsic apoptosis.Frontiers in pharmacology · 2024Article
- Open multi-organ communication device for easy interrogation of tissue slices.Lab on a chip · 2023Article
- Making In Vitro Tumor Models Whole Again.Advanced healthcare materials · 2023Review
- Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics.Nature immunology · 2022Article
- Acute Lymph Node Slices Are a Functional Model System to Study Immunity Ex Vivo.ACS pharmacology & translational science · 2021Article
- Labeling and tracking of immune cells in ex vivo human skin.Nature protocols · 2021Article
- Spatially resolved measurement of dynamic glucose uptake in live ex vivo tissues.Analytica chimica acta · 2021Article
- Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction.Frontiers in veterinary science · 2021Review
- Spatially Resolved Analytical Chemistry in Intact, Living Tissues.Analytical chemistry · 2020Review
- Labelling primary immune cells using bright blue fluorescent nanoparticles.Biomaterials science · 2020Article
- Optimization of Enzymatic Antibody Fragmentation for Yield, Efficiency, and Binding Affinity.Bioconjugate chemistry · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Explants of lymphoid tissue provide a rare opportunity to assess the organization of the immune system in a living, dynamic environment. Traditionally, ex vivo immunostaining is conducted in fixed tissue sections, while live tissues are analyzed using genetically engineered fluorescent reporters or adoptively transferred, pre-labelled cell populations. Here, we validated a protocol for immunostaining and imaging in live, thick slices of lymph node tissue, thus providing a spatial "map" of the lymph node while maintaining the viability and functionality of the slices. Using anti-B220/CD45R (B cell) as a prototype antibody, the procedure for immunostaining was tested for sufficient signal to noise with respect to staining time, temperature, and wash time, and the specificity was verified in comparison to isotype controls. Immunostaining signal in live tissue slices was detectable to atleast 120 μm deep for both whole antibodies and F(ab')
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.