Evidence map›Paper›PMID 30343040›Full record

ArticleExperimental eye research2019

Impact of topical corticosteroid pretreatment on susceptibility of the injured murine cornea to Pseudomonas aeruginosa colonization and infection.

Yvonne T Wu, Tan N Truong, Connie Tam, Myra N Mendoza, Lucia Zhu, David J Evans, Suzanne M J Fleiszig

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Effect of preoperative eyedrops on cytokine concentrations in aqueous humor of patients undergoing femtosecond laser-assisted cataract surgery.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2022
    Trial
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yvonne T WuSchool of Optometry, University of California, Berkeley, CA, USA.
Tan N TruongSchool of Optometry, University of California, Berkeley, CA, USA; Vision Science Program, University of California, Berkeley, CA, USA.
Connie TamSchool of Optometry, University of California, Berkeley, CA, USA.
Myra N MendozaSchool of Optometry, University of California, Berkeley, CA, USA.
Lucia ZhuSchool of Optometry, University of California, Berkeley, CA, USA.
David J EvansSchool of Optometry, University of California, Berkeley, CA, USA; College of Pharmacy, Touro University California, Vallejo, CA, USA.
Suzanne M J FleiszigSchool of Optometry, University of California, Berkeley, CA, USA; Vision Science Program, University of California, Berkeley, CA, USA; Graduate Groups in Microbiology, And Infectious Diseases & Immunity, University of California, Berkeley, CA, USA. Electronic address: fleiszig@berkeley.edu.
University of California, Berkeley · US

Funding

UC Berkeley Vision Science COREP30EY003176 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI KARSTEN GRONERT · 1985 to 2026
$16.4M
Berkeley Clinical Scientist Development ProgramK12EY017269 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI POLSE, KENNETH A · 2005 to 2014
$8.6M
SIGNIFICANCE OF CORNEAL CELL INVASION BY BACTERIAR01EY011221 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI Suzanne MJ FLEISZIG · 1995 to 2026
$8.4M
Cytoskeletal Keratins in Epithelial Immunity to Bacterial KeratitisR01EY023000 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI TAM, K. P. CONNIE · 2013 to 2017
$1.8M
NEI NIH HHS K12 EY017269NEI NIH HHS P30 EY003176NEI NIH HHS R01 EY011221NEI NIH HHS R01 EY023000
6 · The paper itself

Abstract

Research with animal models of Pseudomonas aeruginosa keratitis has shown that use of a topical corticosteroid alone against an established infection can significantly increase the number of colonizing bacteria or worsen clinical disease. Moreover, retrospective analysis has suggested that corticosteroid use in humans is associated with an increased risk of keratitis in eyes with pre-existing disease. Thus, while corticosteroids are often used to reduce ocular inflammation in the absence of infection, the risk of opportunistic infection remains a concern. However, the effect of corticosteroids on the intrinsic barrier function of uninfected corneas is unknown. Here, we tested if short-term topical corticosteroid treatment of an uninfected murine cornea would increase susceptibility to P. aeruginosa colonization or infection after epithelial injury. Topical prednisolone acetate (1%) was administered to one eye of C57BL/6 mice three times a day for 3 days; control eyes were treated with sterile PBS. Prior to inoculation with a cytotoxic P. aeruginosa corneal isolate strain 6206, corneas were subject to superficial-injury by tissue paper blotting, or scratch-injured followed by 12 h of healing. Previously we have shown that blotting renders mouse corneas susceptible to P. aeruginosa adhesion, but not infection, while 12 h healing reduces susceptibility to infection after scratching. Corneas were evaluated at 48 h for bacterial colonization and microbial keratitis (MK). To monitor impact on wound healing, corneal integrity was examined by fluorescein staining immediately after scarification and after 12 h healing. For both the tissue paper blotting and scratch-injury models, there was no significant difference in P. aeruginosa colonization at 48 h between corticosteroid-pretreated eyes and controls. With the blotting model, one case of MK was observed in a control (PBS-pretreated) cornea; none in corticosteroid-pretreated corneas. With the 12 h healing model, MK occurred in 6 of 17 corticosteroid-pretreated eyes versus 2 of 17 controls, a difference not statistically significant. Corticosteroid-pretreated eyes showed greater fluorescein staining 12 h after scarification injury, but this did not coincide with increased colonization or MK. Together, these data show that short-term topical corticosteroid therapy on an uninfected murine cornea does not necessarily enhance its susceptibility to P. aeruginosa colonization or infection after injury, even when it induces fluorescein staining.

Indexed as

Administration, OphthalmicAnimalsCorneaCorneal InjuriesCorneal UlcerDisease Models, AnimalDisease SusceptibilityEpithelium, CornealEye Infections, BacterialFemaleFluoresceinFluorescent DyesGlucocorticoidsMiceMice, Inbred C57BLPrednisoloneFluoresceinFluorescent DyesGlucocorticoidsPrednisoloneprednisolone acetateBacterial colonizationCorticosteroid pretreatmentEpithelial healingMicrobial keratitisMurine corneaPseudomonas aeruginosaSuperficial injury

Identifiers

PMID30343040
PMCPMC6360113
OpenAlexW2897593777

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.