ArticleBiochimica et biophysica acta. General subjects2019
Catechin-mediated restructuring of a bacterial toxin inhibits activity.
Article in Biochimica et biophysica acta. General subjects, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
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- Toxin-triggered liposomes for the controlled release of antibiotics to treat infections associated with the gram-negative bacterium, Aggregatibacter actinomycetemcomitans.Colloids and surfaces. B, Biointerfaces · 2024Article
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- Potential of phenolic compounds from pomegranate (Saudi journal of biological sciences · 2023Review
- Antimicrobial Activity of Quercetin, Naringenin and Catechin: Flavonoids InhibitMolecules (Basel, Switzerland) · 2023Article
- Green Tea-Derived Catechins Suppress the Acid Productions of Streptococcus mutans and Enhance the Efficiency of Fluoride.Caries research · 2023Article
- Advances in novel therapeutic approaches for periodontal diseases.BMC oral health · 2022Review
- Review
- Virtual Screening of Natural Chemical Databases to Search for Potential ACE2 Inhibitors.Molecules (Basel, Switzerland) · 2022Article
- Effects of green tea extract epigallocatechin-3-gallate (EGCG) on oral disease-associated microbes: a review.Journal of oral microbiology · 2022Review
- Aggregatibacter actinomycetemcomitans: From Basic to Advanced Research.Advances in experimental medicine and biology · 2022Article
- Matcha Green Tea Exhibits Bactericidal Activity againstAntibiotics (Basel, Switzerland) · 2021Article
- Prophylactic Catechin-Rich Green Tea Extract Treatment Ameliorates Pathogenic EnterotoxicPathogens (Basel, Switzerland) · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCatechins, polyphenols derived from tea leaves, have been shown to have antibacterial properties, through direct killing of bacteria as well as through inhibition of bacterial toxin activity. In particular, certain catechins have been shown to have bactericidal effects on the oral bacterium, Aggregatibacter actinomycetemcomitans, as well as the ability to inhibit a key virulence factor of this organism, leukotoxin (LtxA). The mechanism of catechin-mediated inhibition of LtxA has not been shown.
methodsIn this work, we studied the ability of six catechins to inhibit LtxA-mediated cytotoxicity in human white blood cells, using Trypan blue staining, and investigated the mechanism of action using a combination of techniques, including fluorescence and circular dichroism spectroscopy, confocal microscopy, and surface plasmon resonance.
resultsWe found that all the catechins except (-)-catechin inhibited the activity of this protein, with the galloylated catechins having the strongest effect. Pre-incubation of the toxin with the catechins increased the inhibitory action, indicating that the catechins act on the protein, rather than the cell. The secondary structure of LtxA was dramatically altered in the presence of catechin, which resulted in an inhibition of toxin binding to cholesterol, an important initial step in the cytotoxic mechanism of the toxin.
conclusionsThese results demonstrate that the catechins inhibit LtxA activity by altering its structure to prevent interaction with specific molecules present on the host cell surface. GENERAL SIGNIFICANCE: Galloylated catechins modify protein toxin structure, inhibiting the toxin from binding to the requisite molecules on the host cell surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.