ArticleMolecular biology reports2018
Role of Spata34 in cell proliferation and its expression pattern in postnatal development of rat testis.
Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 1 citations in OpenAlex.
- The testis-specific expressed gene Spata34 is not required for fertility in mice.Molecular biology reports · 2020Article
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4 authors at 2 institutions in 1 country.
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Abstract
Spata34 is a testis-specific-expressed gene which exerts diverse functions in testis development. This study intends to examine the expression profiles of Spata34 in postnatal rat testis, and explore its potential roles in cell proliferation in vitro. We found that the mRNA and protein expression levels of Spata34 were developmentally upregulated in rat testes during the early 1-7 postnatal weeks using real-time polymerase chain reaction and western blotting. Immunohistochemical results indicated that Spata34 protein was mainly detected in the nuclear and cytoplasm of spermatocytes and round spermatids. The possible function of Spata34 in cellular proliferation was analyzed using cell counting kit, colony formation and flow cytometry assays. Our results showed that overexpression of Spata34 in multipotent adult germline stem cell lines (maGSC129SV) cells markedly facilitated cell proliferation with a large increase in cell numbers in S phase of cell cycle. While knockdown of Spata34 expression by specific siRNA suppressed the growth of maGSC129SV cells and triggered cell-cycle arrest at G1/S phase transition, which was related to the elevation of p21 and p27 and decrease of Cyclin D1 and Cyclin D-dependent kinase 4. Altogether, our results indicated that the Spata34 gene evokes unique expression patterns during postnatal development of the rat testis, and for the first time, unravels the function of Spata34 on regulating cell-cycle progress through p21 and p27 pathway.
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