Evidence map›Paper›PMID 30341632›Full record

ArticleIn vitro cellular & developmental biology. Animal2018

Short-term in vitro culture of purity and highly functional rat bone marrow-derived mast cells.

Tianyu Yu, Bin Liu, Zhigang He, Muqing Yang, Jian Song, Cheng Ma, Sunqiang Ma, Junlan Feng, Xiaodong Wang, Jiyu Li

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Tianyu YuDepartment of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, 81 MeShan Road, Hefei, 230032, Anhui, People's Republic of China.
Bin LiuDepartment of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, 81 MeShan Road, Hefei, 230032, Anhui, People's Republic of China.
Zhigang HeDepartment of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, People's Republic of China.
Muqing YangDepartment of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, People's Republic of China.
Jian SongDepartment of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, People's Republic of China.
Cheng MaDepartment of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, 81 MeShan Road, Hefei, 230032, Anhui, People's Republic of China.
Sunqiang MaDepartment of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, People's Republic of China.
Junlan FengDepartment of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, People's Republic of China.
Xiaodong WangDepartment of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, 81 MeShan Road, Hefei, 230032, Anhui, People's Republic of China.
Jiyu LiDepartment of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, 81 MeShan Road, Hefei, 230032, Anhui, People's Republic of China. leejiyu@sina.com.ORCID http://orcid.org/0000-0003-2560-1016
Tongji University · CN

Funding

National Natural Science Foundation of China 31741087National Natural Science Foundation of China 81470897
6 · The paper itself

Abstract

Mast cells (MCs) are responsible for the innate immune response. Rat MCs are more suitable than mouse MCs as models of specific parasite infection processes and ovalbumin-induced asthma. Rat peritoneum-derived MCs and RBL-2H3 cells (an MC cell line) are widely used in disease studies. However, the application of rat bone marrow-derived MCs (BMMCs) are poorly documented in terms of the methodology of rat BMMC isolation. Here, we describe a relatively rapid, efficient, and simple method for the cultivation of rat BMMCs. As compared to previous protocols, rat BMMCs produced with the proposed protocol exhibited advantages in differentiation, proliferation, lifespan, and functionality, which should prove useful for studies of mucosal MC diseases in specific rat models.

Indexed as

Animalsbeta-N-AcetylhexosaminidasesBone Marrow CellsCell Culture TechniquesCell DegranulationCells, CulturedCell SeparationChymasesHistamineMaleMast CellsPeptide HydrolasesRats, Sprague-DawleyRNA, Messengerbeta-N-Acetylhexosaminidaseschymase 2ChymasesHistamineMcpt1 protein, ratPeptide HydrolasesRNA, MessengerAA4Bone marrow-derived mast cellDifferentiationFcεRIIn vitroPurificationRat

Identifiers

PMID30341632
OpenAlexW2897928977

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.