ReviewWorld journal of microbiology & biotechnology2018
Wanted: more monitoring and control during inclusion body processing.
Review in World journal of microbiology & biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Engineering Microbial Particles for Next-Generation Biomedical Platforms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence.Analytical and bioanalytical chemistry · 2024Article
- Fundamental insights in early-stage inclusion body formation.Microbial biotechnology · 2023Article
- Optimized Signal Peptide for Secretory Expression of Human Recombinant Somatropin inAdvanced pharmaceutical bulletin · 2023Article
- Structure-Activity Relationship of the Dimeric and Oligomeric Forms of a Cytotoxic Biotherapeutic Based on Diphtheria Toxin.Biomolecules · 2022Article
- Sandwich-Based Immunosensor for Dual-Mode Detection of Pathogenic F17-PositiveInternational journal of molecular sciences · 2022Article
- The N2A region of titin has a unique structural configuration.The Journal of general physiology · 2021Article
- At-Line Reversed Phase Liquid Chromatography for In-Process Monitoring of Inclusion Body Solubilization.Bioengineering (Basel, Switzerland) · 2021Article
- Advances in monitoring and control of refolding kinetics combining PAT and modeling.Applied microbiology and biotechnology · 2021Review
- Scalable High-Performance Production of Recombinant Horseradish Peroxidase fromInternational journal of molecular sciences · 2020Article
- Construction and Validation of a Protein Prognostic Model for Lung Squamous Cell Carcinoma.International journal of medical sciences · 2020Article
- Perspectives of inclusion bodies for bio-based products: curse or blessing?Applied microbiology and biotechnology · 2019Review
- Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process.Microbial cell factories · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Inclusion bodies (IBs) are insoluble aggregates of misfolded protein in Escherichia coli. Against the outdated belief that the production of IBs should be avoided during recombinant protein production, quite a number of recombinant products are currently produced as IBs, which are then processed to give correctly folded and soluble product. However, this processing is quite cumbersome comprising IB wash, IB solubilization and refolding. To date, IB processing often happens rather uncontrolled and relies on empiricism rather than sound process understanding. In this mini review we describe current efforts to introduce more monitoring and control in IB processes, focusing on the refolding step, and thus generate process understanding and knowledge.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.