Evidence map›Paper›PMID 30341238›Full record

ArticleBioscience reports2018

Indirubin attenuates mouse psoriasis-like skin lesion in a CD274-dependent manner: an achievement of RNA sequencing.

Xiaochun Xue, Jianhua Wu, Junhui Li, Jianguo Xu, Haiying Dai, Congshan Tao, Chao Li, Jinhong Hu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Xiaochun XueDepartment of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Jianhua WuDepartment of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Junhui LiDepartment of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Jianguo XuDepartment of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Haiying DaiDepartment of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Congshan TaoDepartment of Pharmacy, Wuxi Maternal and Child Health-Care Hospital, Wuxi 214000, China.
Chao LiDepartment of Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China.
Jinhong HuDepartment of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China jinhonghu2018@163.com.ORCID 0000-0002-9218-4673
Second Military Medical University · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It was previously reported that the expression of CD274 was down-regulated in psoriatic epidermis, leading to immune disorders of psoriasis. However, the regulatory mechanisms of CD274 were rarely elucidated. We aimed to explore the regulatory mechanisms of CD274. Skin samples were collected from 18 patients with psoriasis vulgaris and 9 healthy participants for RNA sequencing. Candidate genes were chosen based on degree and k-core difference of genes in the co-expression network. The relations between candidate genes and CD274 were validated by flow cytometry and real-time PCR in primary human epidermal keratinocytes. The therapeutic effect of indirubin was assessed in an imiquimod-treated mouse model. Interferon-γ (IFN-γ), cyclin-dependent kinase (CDK) 1, Toll-like receptor 3 (TLR3), TLR4 and interleukin (IL)-17A were considered as candidate genes. In primary human epidermal keratinocytes, the level of CD274 was obviously increased under the stimulation of IFN-γ and CDK1 inhibitor (indirubin), independent of TLR4, TLR3 or IL-17A. Indirubin alleviated the severity of psoriatic mice in a CD274-dependent manner. Co-expression network analysis served as an effective method for the exploration of molecular mechanisms. We demonstrated for the first time that CD274 was the regulator of indirubin-mediated effect on mouse psoriasis-like skin lesion based on co-expression network analysis, contributing to the alleviation of mouse psoriasis-like skin lesion.

Indexed as

AdolescentAdultAgedAnimalsB7-H1 AntigenCDC2 Protein KinaseDisease Models, AnimalEpidermisFemaleFlow CytometryGene Expression RegulationHumansIndolesInterferon-gammaInterleukin-17KeratinocytesB7-H1 AntigenCd274 protein, mouseCDC2 Protein KinaseIFNG protein, humanIL17A protein, humanindirubinIndolesInterferon-gammaInterleukin-17TLR3 protein, humanTLR4 protein, humanToll-Like Receptor 3Toll-Like Receptor 4differentially expressed genesIFN-γindirubinPD-L1psoriasisRNA sequencing

Identifiers

PMID30341238
PMCPMC6250808
OpenAlexW2896129332

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.