ArticleCell2018
Real-Time Genetic Compensation Defines the Dynamic Demands of Feedback Control.
Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 98 citations in OpenAlex.
- Dynamic heterogeneity in an E. coli stress response regulon mediates gene activation and antimicrobial peptide tolerance.Cell reports · 2026Article
- Single-cell analysis and control of microbial systems using optogenetics.Current opinion in microbiology · 2026Review
- Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.Nature communications · 2025Article
- Lighting up yeast: overview of optogenetics in yeast and their applications to yeast biotechnology.FEMS yeast research · 2025Review
- Article
- Yeast cell responses and survival during periodic osmotic stress are controlled by glucose availability.eLife · 2024Article
- Light-inducible protein degradation ineLife · 2024Article
- High-throughput feedback-enabled optogenetic stimulation and spectroscopy in microwell plates.Communications biology · 2023Article
- Quantitative insights in tissue growth and morphogenesis with optogenetics.Physical biology · 2023Review
- Protein dynamics provide mechanistic insights about epistasis among common missense polymorphisms.Biophysical journal · 2023Article
- Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit.ACS synthetic biology · 2023Article
- Determining growth rates from bright-field images of budding cells through identifying overlaps.eLife · 2023Article
- Synthetic developmental biology: New tools to deconstruct and rebuild developmental systems.Seminars in cell & developmental biology · 2023Review
- Coupling Cell Communication and Optogenetics: Implementation of a Light-Inducible Intercellular System in Yeast.ACS synthetic biology · 2023Article
- CyberSco.Py an open-source software for event-based, conditional microscopy.Scientific reports · 2022Article
- Enhancing bioreactor arrays for automated measurements and reactive control with ReacSight.Nature communications · 2022Article
- Enabling reactive microscopy with MicroMator.Nature communications · 2022Article
- Article
- Platforms for Optogenetic Stimulation and Feedback Control.Frontiers in bioengineering and biotechnology · 2022Review
- Functional network motifs defined through integration of protein-protein and genetic interactions.PeerJ · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Biological signaling networks use feedback control to dynamically adjust their operation in real time. Traditional static genetic methods such as gene knockouts or rescue experiments can often identify the existence of feedback interactions but are unable to determine what feedback dynamics are required. Here, we implement a new strategy, closed-loop optogenetic compensation (CLOC), to address this problem. Using a custom-built hardware and software infrastructure, CLOC monitors, in real time, the output of a pathway deleted for a feedback regulator. A minimal model uses these measurements to calculate and deliver-on the fly-an optogenetically enabled transcriptional input designed to compensate for the effects of the feedback deletion. Application of CLOC to the yeast pheromone response pathway revealed surprisingly distinct dynamic requirements for three well-studied feedback regulators. CLOC, a marriage of control theory and traditional genetics, presents a broadly applicable methodology for defining the dynamic function of biological feedback regulators.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.