ArticlePhysiological reports2018
Repressors of mTORC1 act to blunt the anabolic response to feeding in the soleus muscle of a cast-immobilized mouse hindlimb.
Article in Physiological reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
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- Use of Amino Acids as Supplements for Matching Nutrition, Training, and Rehabilitation-Focusing on Some Questions.Nutrients · 2025Review
- Evaluating skeletal muscle wasting and weakness in models of critical illness.Clinical science (London, England : 1979) · 2025Review
- The Effect of Cachexia on the Feeding Regulation of Skeletal Muscle Protein Synthesis in Tumour-Bearing Mice.Journal of cachexia, sarcopenia and muscle · 2025Article
- Dietary RNA from Torula Yeast Prevents Capillary Regression in Atrophied Skeletal Muscle in Rats.Life (Basel, Switzerland) · 2024Article
- Article
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- Nutritional Supplementation and Exercise as Essential Allies in the Treatment of Chronic Heart Failure: The Metabolic and Molecular Bases.Nutrients · 2023Review
- Pathophysiological Aspects of Muscle Atrophy and Osteopenia Induced by Chronic Constriction Injury (CCI) of the Sciatic Nerve in Rats.International journal of molecular sciences · 2023Article
- Loss of 4E-BPs prevents the hindlimb immobilization-induced decrease in protein synthesis in skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- AKT controls protein synthesis and oxidative metabolism via combined mTORC1 and FOXO1 signalling to govern muscle physiology.Journal of cachexia, sarcopenia and muscle · 2022Article
- Amino Acid Trafficking and Skeletal Muscle Protein Synthesis: A Case of Supply and Demand.Frontiers in cell and developmental biology · 2021Review
- A time course for markers of protein synthesis and degradation with hindlimb unloading and the accompanying anabolic resistance to refeeding.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Cachexia Disrupts Diurnal Regulation of Activity, Feeding, and Muscle Mechanistic Target of Rapamycin Complex 1 in Mice.Medicine and science in sports and exercise · 2020Article
- Repressors of mTORC1 act to blunt the anabolic response to feeding in the soleus muscle of a cast-immobilized mouse hindlimb.Physiological reports · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We recently reported results showing that cast immobilization of a rat hindlimb rapidly leads to development of anabolic resistance as demonstrated by failure of oral leucine administration to activate the mechanistic target of rapamycin complex 1 (mTORC1) and stimulate protein synthesis in the soleus muscle. The goal of this study was to assess the possible contribution of several mTORC1 regulatory proteins to the development of anabolic resistance. To accomplish this, 14-week-old male C57BL/6 mice (n = 21) were subjected to unilateral cast immobilization of the hindlimb for either 1 or 3 days, and the immobilized limb was compared to its contralateral control. The mass of the soleus muscle was decreased in the immobilized compared to the non-immobilized limb within 72-h in association with diminished protein synthesis. In agreement with our previous report, a 24-h casting period was sufficient to induce anabolic resistance, as demonstrated by blunted re-feeding-induced activation of mTORC1. Moreover, resistance of mTORC1 activation was associated not only with upregulated expression of REDD1, but also with altered expression of other mTORC1 regulatory proteins, that is, Sestrin1 and DEP domain-containing mTOR interacting protein (DEPTOR). In addition, re-feeding-induced phosphorylation of DEPTOR was significantly impaired in the immobilized compared to the non-immobilized limb. This work builds upon previous discoveries by our laboratory to elucidate the blunted mTORC1 response to stimuli during disuse of skeletal muscle induced by cast immobilization while highlighting new potential therapeutic targets for future countermeasures against muscle atrophy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.