ArticleBiomedical optics express2018
Hyperspectral imaging in highly scattering media by the spectral phasor approach using two filters.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Phasor-Based Spatio-Spectral Segmentation for Hyperspectral Fluorescence Microscopy.Journal of biophotonics · 2026Article
- PolSpec : Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging.Journal of biophotonics · 2025Article
- High-Speed Hyperspectral Imaging for Near Infrared Fluorescence and Environmental Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.Biomedical optics express · 2025Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.Cell reports methods · 2023Article
- Multiplexed bioluminescence microscopy via phasor analysis.Nature methods · 2022Article
- Method of transmission filters to measure emission spectra in strongly scattering media.Biomedical optics express · 2021Article
- Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications.Communications biology · 2021Article
- Application of Microspectral Imaging in Motor and Sensory Nerve Classification.Journal of healthcare engineering · 2021Article
- The DIVER Microscope for Imaging in Scattering Media.Methods and protocols · 2019Review
- A metabolic interpretation for the response of cellular autofluorescence to chemical perturbations assessed using spectral phasor analysis.RSC advances · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Hyperspectral imaging is a common technique in fluorescence microscopy to obtain the emission spectrum at each pixel of an image. However, methods to obtain spectral resolution based on diffraction gratings or integrated prisms work poorly when the sample is strongly scattering. We developed a microscope named the DIVER that collects the fluorescence emission over a very large angle. Since the fluorescence light after passing through the multiple scattering sample is not collimated, the use of grating or prisms strongly limits the amount of light that can be used with available hyperspectral devices. Here we show that 2 filters that accept uncollimated light over a large aperture are sufficient to calculate the spectral phasor rather than displaying the entire spectrum. Using the properties of the spectral phasors, we can resolve spectral components and perform the type of data analyses that are usually performed in hyperspectral image analysis.
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Registered trials
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