ArticleOpen access Macedonian journal of medical sciences2018
Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites.
Article in Open access Macedonian journal of medical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children.Parasitology research · 2023Article
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3 authors.
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Abstract
background
aimThe present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples.
methodsThe target DNA was provided in 8 different quantities.
resultsAmplification failure was reported only with the cPCR in samples of low concentrations using both primer sets. Quantitative PCR detected the 8 different dilutions of the purified
conclusionGenerally real-time quantitative molecular assays, is easy to use method compared to conventional PCR assay and produces more reliable results within only one hour time but still the possible application of qPCRs in routine diagnosis necessitates analysis of a large number of clinical samples in further studies to make the proper choice.
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