ArticleGut pathogens2018
Purification of
Article in Gut pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Evaluation of nucleic acid extraction methods for recovery of Cyclospora cayetanensis, Salmonella enterica, and murine norovirus from water and sludge.Journal of microbiological methods · 2025Article
- Evaluation of the Increased Genetic Resolution and Utility for Source Tracking of a Recently Developed Method for GenotypingMicroorganisms · 2024Article
- Evaluation of nuclear and mitochondrial phylogenetics for the subtyping of Cyclospora cayetanensis.Parasitology research · 2023Article
- The limit of detection of the BioFire® FilmArray® gastrointestinal panel for the foodborne parasite Cyclospora cayetanensis.Diagnostic microbiology and infectious disease · 2023Article
- Article
- Targeted next generation sequencing ofHeliyon · 2022Article
- Hastening Progress inMicroorganisms · 2022Article
- Genotyping CanadianMicroorganisms · 2022Article
- Detection ofFood and waterborne parasitology · 2021Article
- Dynamically expressed genes provide candidate viability biomarkers in a model coccidian.PloS one · 2021Article
- Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing.Parasites & vectors · 2020Article
- Advances in Cyclosporiasis Diagnosis and Therapeutic Intervention.Frontiers in cellular and infection microbiology · 2020Review
- Development of a workflow for identification of nuclear genotyping markers for Cyclospora cayetanensis.Parasite (Paris, France) · 2020Article
- Pathogen Genomics in Public Health.The New England journal of medicine · 2019Article
- Review
- Genotyping genetically heterogeneousParasitology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background
resultsOocysts from 21 human stool specimens were separated from other stool components using discontinuous density gradient centrifugation and purified further by flow cytometry. Genomic DNA was used to construct Ovation Ultralow libraries for Illumina sequencing. MiSeq sequencing reads were taxonomically profiled for contamination, de novo assembled, and mapped to a draft genome available in GenBank to assess the quality of the resulting genomic sequences. Following all purification steps, the majority (81-99%) of sequencing reads were from
conclusionsDensity gradients performed in the presence of a detergent followed by flow cytometry sorting of oocysts yielded sufficient genomic DNA largely free from contamination and suitable for whole genome sequencing of
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.