ArticlePlant methods2018
Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Genome-wide identification and expression profiling of the bHLH transcription factor family under salt stress and hormonal treatments in cowpea (Vigna unguiculata).Scientific reports · 2026Article
- Identification and expression analysis of the Xyloglucan Transglycosylase/Hydrolase (XTH) gene family under salt stress in cowpea (Vigna unguiculata L.).BMC genomics · 2026Article
- Article
- Combining the CowPEAsy Web Application With in Planta Agroinfiltration for Native Promoter Validation in Vigna unguiculata.Plant, cell & environment · 2025Article
- Salt-Resilient Cowpeas: Early Identification Through Growth Parameters and Gene Expression at Germination Stage.International journal of molecular sciences · 2025Article
- Cowpea (Frontiers in plant science · 2025Review
- Using WGCNA and transcriptome profiling to identify hub genes for salt stress tolerance in germinating soybean seeds.Frontiers in plant science · 2025Article
- Crop Wild Relatives (CWRs) in the United Arab Emirates: Resources for Climate Resilience and Their Potential Medicinal Applications.Drug design, development and therapy · 2025Review
- Genome-Wide Identification and Stress Responses of Cowpea Thaumatin-like Proteins: A Comprehensive Analysis.Plants (Basel, Switzerland) · 2024Article
- Contrast Relative Humidity Response of Diverse Cowpea (International journal of molecular sciences · 2024Article
- Genome-wide analysis and expression profile of3 Biotech · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.Scientific reports · 2024Article
- Flavonoids are involved in salt tolerance through ROS scavenging in the halophyte Atriplex canescens.Plant cell reports · 2023Article
- The Content of Anthocyanins in Cowpea (Plants (Basel, Switzerland) · 2023Article
- Evaluating and Validating Sunflower Reference Genes for Q-PCR Studies Under High Temperature Condition.Iranian journal of biotechnology · 2023Article
- Cowpea Constraints and Breeding in Europe.Plants (Basel, Switzerland) · 2023Review
- Transcriptome Analysis of Differentially Expressed Genes Associated with Salt Stress in Cowpea (International journal of molecular sciences · 2023Article
- Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses.Plants (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDue to cowpea ability to fix nitrogen in poor soils and relative tolerance to drought and salt stresses, efforts have been directed to identifying genes and pathways that confer stress tolerance in this species. Real-time quantitative PCR (qPCR) has been widely used as the most reliable method to measure gene expression, due to its high accuracy and specificity. In the present study, nine candidate reference genes were rigorously tested for their application in normalization of qPCR data onto roots of four distinct cowpea accessions under two abiotic stresses: root dehydration and salt (NaCl, 100 mM). In addition, the regulation of four target transcripts, under the same referred conditions was also scrutinized.
resultsgeNorm, NormFinder, BestKeeper, and ΔCt method results indicated a set of three statistically validated RGs for each stress condition: (I) root dehydration (actin, ubiquitin-conjugating enzyme E2 variant 1D, and a
conclusionsReference genes suitable for qPCR analyses in cowpea under root dehydration and salt stress were identified. This action will lead to a more accurate and reliable analysis of gene expression on this species. Additionally, the results obtained in this study may guide future research on gene expression in cowpea under other abiotic stress types that impose osmotic imbalance. The target genes analyzed, in turn, deserve functional evaluation due to their transcriptional regulation under stresses and biotechnological potential.
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