ArticleFrontiers in plant science2018
Ectopic Transplastomic Expression of a Synthetic MatK Gene Leads to Cotyledon-Specific Leaf Variegation.
Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Structural bioinformatics and gene expression analysis of maturase K fromFrontiers in molecular biosciences · 2025Article
- Transgene insertion into the plastid genome alters expression of adjacent native chloroplast genes at the transcriptional and translational levels.Plant biotechnology journal · 2023Article
- Plastome evolution in the East Asian lobelias (Lobelioideae) using phylogenomic and comparative analyses.Frontiers in plant science · 2023Article
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- Plastid genome evolution in Amazonian açaí palm (Euterpe oleracea Mart.) and Atlantic forest açaí palm (Euterpe edulis Mart.).Plant molecular biology · 2021Article
- EndophyticFrontiers in microbiology · 2021Article
- Unraveling the role of the enigmatic MatK maturase in chloroplast group IIA intron excision.Plant direct · 2020Article
- Chloroplast Genome Sequences and Comparative Analyses of Combretaceae Mangroves with Related Species.BioMed research international · 2020Article
- Multifarious Evolutionary Pathways of a Nuclear RNA Editing Factor: Disjunctions in Coevolution of DOT4 and Its Chloroplast Target rpoC1eU488SL.Genome biology and evolution · 2019Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts (and other plastids) harbor their own genetic material, with a bacterial-like gene-expression systems. Chloroplast RNA metabolism is complex and is predominantly mediated by nuclear-encoded RNA-binding proteins. In addition to these nuclear factors, the chloroplast-encoded intron maturase MatK has been suggested to perform as a splicing factor for a subset of chloroplast introns. MatK is essential for plant cell survival in tobacco, and thus null mutants have not yet been isolated. We therefore attempted to over-express MatK from a neutral site in the chloroplast, placing it under the control of a theophylline-inducible riboswitch. This ectopic insertion of MatK lead to a variegated cotyledons phenotype. The addition of the inducer theophylline exacerbated the phenotype in a concentration-dependent manner. The extent of variegation was further modulated by light, sucrose and spectinomycin, suggesting that the function of MatK is intertwined with photosynthesis and plastid translation. Inhibiting translation in the transplastomic lines has a profound effect on the accumulation of several chloroplast mRNAs, including the accumulation of an RNA antisense to
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