ArticleScientific reports2018
HPV16-Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Ectocervical margin involvement is associated with postoperative hrHPV positivity after hysterectomy or residual cervix resection for incompletely excised CIN3: a retrospective study.BMC women's health · 2026Article
- Review
- Defining precancer: a grand challenge for the cancer community.Nature reviews. Cancer · 2024Review
- Fibroblast stromal support model for predicting human papillomavirus-associated cancer drug responses.Journal of virology · 2024Article
- Organoid modeling meets cancers of female reproductive tract.Cell death discovery · 2024Review
- Cervical cancer in Thailand: 2023 update.Obstetrics & gynecology science · 2024Article
- Human papillomavirus associated cervical lesion: pathogenesis and therapeutic interventions.MedComm · 2023Review
- Advanced Technologies for Studying Microbiome-Female Reproductive Tract Interactions: Organoids, Organoids-on-a-Chip, and Beyond.Seminars in reproductive medicine · 2023Review
- Confronting the human papillomavirus-HIV intersection: Cervical cytology implications for Kenyan women living with HIV.Southern African journal of HIV medicine · 2023Article
- Notch signaling sculpts the stem cell niche.Frontiers in cell and developmental biology · 2022Review
- RNA Sequencing and Cell Models of Virus-Associated Cancer (Review).Sovremennye tekhnologii v meditsine · 2022Review
- Establishment of a Three-Dimensional In Vitro Model of Equine Papillomavirus Type 2 Infection.Viruses · 2021Article
- Human papillomavirus E6 and E7: What remains?Tumour virus research · 2021Review
- Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.Nature cell biology · 2021Article
- Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes.Frontiers in cell and developmental biology · 2021Review
- Organoid systems to study the human female reproductive tract and pregnancy.Cell death and differentiation · 2021Review
- In Vitro Organotypic Systems to Model Tumor Microenvironment in Human Papillomavirus (HPV)-Related Cancers.Cancers · 2020Review
- Telomerase Reverse Transcriptase (TERT) Expression, Telomerase Activity, and Expression of Matrix Metalloproteinases (MMP)-1/-2/-9 in Feline Oral Squamous Cell Carcinoma Cell Lines Associated WithFrontiers in veterinary science · 2020Article
- Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures.Journal of cellular physiology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A major risk factor for cervical cancer is persistent infection with high-risk human papillomaviruses (HPV) which can cause cervical intraepithelial neoplasia. Greater than 90% of cervical cancers develop in the transformation zone (TZ), a small region of metaplastic squamous epithelium at the squamocolumnar junction between endocervix and ectocervix. However, it is unclear why this region is highly susceptible to malignant progression. We hypothesized that cells from TZ were more susceptible to dysplastic differentiation, a precursor to cervical cancer. We used three-dimensional organotypic culture to compare differentiation of HPV16-immortalized epithelial cell lines derived from ectocervix, TZ, and endocervix. We show that immortal cells from TZ or endocervix form epithelia that are more dysplastic than immortal cells from ectocervix. A higher percentage of immortal cells from TZ and endocervix express the proliferation marker Ki-67 and are positive for phospho-Akt. Immortal cells from TZ and endocervix invade collagen rafts and express increased levels of matrix metalloproteinase-1. Inhibition of MMP-1 or Akt activity blocks invasion. We conclude that HPV16-immortalized cells cultured from TZ or endocervix are more susceptible to dysplastic differentiation, and this might enhance their susceptibility to cervical cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.