ArticleScientific reports2018
A Real-Time PCR based assay for determining parasite to host ratio and parasitaemia in the clinical samples of Bovine Theileriosis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Prevalence and determinants of bovine theileriosis in India: a meta-analysis and meta-regression.Tropical animal health and production · 2026Pooled it
- mRNA vaccine design using the proteome ofmSphere · 2025Article
- Development of Nested Polymerase Chain Reaction with Novel Specific Primers for Detection ofAnimals : an open access journal from MDPI · 2023Article
- Multiplex ddPCR: A Promising Diagnostic Assay for Early Detection and Drug Monitoring in Bovine Theileriosis.Pathogens (Basel, Switzerland) · 2023Article
- A Multi-copy Nucleic Acid-Based Diagnostic Test for Bovine Tropical Theileriosis.Acta parasitologica · 2022Article
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- Review
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Serological Survey and Molecular Characterization ofPathogens (Basel, Switzerland) · 2021Article
- Antitheilerial Activity of the Anticancer Histone Deacetylase Inhibitors.Frontiers in microbiology · 2021Article
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- Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes.The journal of venomous animals and toxins including tropical diseases · 2020Article
- Population Genetic Analysis of theFrontiers in microbiology · 2020Article
- First Molecular detection of Theileria annulata in Bangladesh.The Journal of veterinary medical science · 2019Article
- Estimation of the Variance Components in TP53 mRNA Expression in the Rat Lens after in vivo Exposure to Ultraviolet Radiation B.Biomedicine hubArticle
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Theileria annulata is an intracellular parasite that causes active and latent forms of bovine theileriosis. Diagnosis of the disease is primarily based on traditional methods such as microscopy, however, PCR based methods have proven to be superior in the absence of clear disease symptoms. However, diagnosis is difficult in cases of lower parasitaemia by conventional PCR. Hence, a rapid and sensitive method which can detect early infection and low parasite load is required. Therefore, we have developed an absolute quantification based real-time PCR (qPCR) assay. Reference standard curve using recombinant plasmids of a host (hprt) and a parasite gene (tasp) was constructed, and the assay was initially standardised using in vitro T. annulata cell lines. Further, 414 blood samples from suspected theileriosis cases were also evaluated using qPCR. The assay can estimate host to parasite ratios, calculate parasitaemia and treatment effectiveness in the clinical cases of theileriosis. In comparison with the conventional PCR results, 44 additional positive cases were found. Therefore, the assay holds importance in a clinical setting due to its ability to quantify the parasite load in clinical samples. It may be further used in distinguishing active and latent theileriosis infections and detection of drug resistance in the field.
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