ArticleScientific reports2018
Genomics of experimental adaptation of Staphylococcus aureus to a natural combination of insect antimicrobial peptides.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Defensins as natural antimicrobial peptide scaffolds against antimicrobial-resistant pathogens: mechanisms, resistance risks, and translational prospects.Frontiers in cellular and infection microbiology · 2026Review
- Identification and antibacterial activity of a novel antimicrobial peptide attacin from Conogethes punctiferalis.Archives of microbiology · 2025Article
- Uncovering the genetic basis ofiScience · 2025Article
- A Trade-Off Between Antimicrobial Peptide Resistance and Sensitivity to Host Immune Effectors inEvolutionary applications · 2025Article
- Mechanisms of Staphylococcus aureus Antibiotics Resistance Revealed by Adaptive Laboratory Evolution.Current microbiology · 2025Article
- Differential Selection for Survival and for Growth in Adaptive Laboratory Evolution Experiments With Benzalkonium Chloride.Evolutionary applications · 2024Article
- Guarding the walls: the multifaceted roles of Bce modules in cell envelope stress sensing and antimicrobial resistance.Journal of bacteriology · 2024Review
- The evolution of antimicrobial peptide resistance in Pseudomonas aeruginosa is severely constrained by random peptide mixtures.PLoS biology · 2024Article
- Article
- Identification of Genes Associated with Resistance to Persulcatusin, a Tick Defensin fromMicroorganisms · 2024Article
- Alternate Antimicrobial Therapies and Their Companion Tests.Diagnostics (Basel, Switzerland) · 2023Review
- The pharmacokinetic-pharmacodynamic modelling framework as a tool to predict drug resistance evolution.Microbiology (Reading, England) · 2023Article
- Lipids as a key element of insect defense systems.Frontiers in genetics · 2023Review
- Antimicrobial Peptides with Antibacterial Activity against Vancomycin-ResistantInternational journal of molecular sciences · 2021Review
- Article
- Insect antimicrobial peptides: potential weapons to counteract the antibiotic resistance.Cellular and molecular life sciences : CMLS · 2021Review
- The Synergistic Effect of Mud Crab Antimicrobial Peptides Sphistin and SphFrontiers in cellular and infection microbiology · 2020Article
- Antimicrobial Peptide Induced-Stress RendersFrontiers in immunology · 2020Article
- Resistance Evolution Against Antimicrobial Peptides inFrontiers in microbiology · 2020Article
- Probiotics Modulate a Novel Amphibian Skin Defense Peptide That Is Antifungal and Facilitates Growth of Antifungal Bacteria.Microbial ecology · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
Antimicrobial peptides (AMP) are highly conserved immune effectors across the tree of life and are employed as combinations. In the beetle Tenebrio molitor, a defensin and a coleoptericin are highly expressed in vivo after inoculation with S. aureus. The defensin displays strong in vitro activity but no survival benefit in vivo. The coleoptericin provides a survival benefit in vivo, but no activity in vitro. This suggests a potentiating effect in vivo, and here we wanted to investigate the effects of this combination on resistance evolution using a bottom-approach in vitro starting with a combination of two abundant AMPs only. We experimentally evolved S. aureus in the presence of the defensin and a combination of the defensin and coleoptericin. Genome re-sequencing showed that resistance was associated with mutations in either the pmt or nsa operons. Strains with these mutations show longer lag phases, slower Vmax, and nsa mutants reach lower final population sizes. Mutations in the rpo operon showed a further increase in the lag phase in nsa mutants but not in pmt mutants. In contrast, final MICs (minimum inhibitory concentrations) do not differ according to mutation. All resistant lines display AMP but not antibiotic cross-resistance. Costly resistance against AMPs readily evolves for an individual AMP as well as a naturally occurring combination in vitro and provides broad protection against AMPs. Such non-specific resistance could result in strong selection on host immune systems that rely on cocktails of AMPs.
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