ArticlePloS one2018
Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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Who cites it
123 citing papers in PubMed.
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- DNA methylation diversity analysis in the cashew germplasm accessions.Molecular biology reports · 2026Article
- Chromosome-level reference genome of a foundational California native legume, Acmispon strigosus.The Journal of heredity · 2026Article
- A genome assembly of Greene's tuctoria, Tuctoria greenei, an amphibious endemic and endangered California vernal pool grass.The Journal of heredity · 2026Article
- A reference genome for Colusa grass, Neostapfia colusana, a threatened and endangered California vernal pool plant.The Journal of heredity · 2026Article
- A genome assembly of Douglas' meadowfoam, Limnanthes douglasii R. BR.The Journal of heredity · 2026Article
- A chromosome level genome assembly of the marine flowering plant, Torrey's surfgrass (Phyllospadix Torreyi) reveals an exceptionally large Y-chromosome.The Journal of heredity · 2026Article
- "A genome assembly of the California flannelbush, Fremontodendron californicum".The Journal of heredity · 2026Article
- Assembling telomere-to-telomere genomes of Fusarium oxysporum f. sp. lactucae provides a roadmap for studying genome and phenotype evolution.BMC genomics · 2026Article
- A genome assembly of the California poppy, Eschscholzia californica.The Journal of heredity · 2026Article
- The reference genome of a Sierra Nevada endemic, the cut-leaved monkeyflower, Mimulus laciniatus (syn. Erythranthe lacinata).The Journal of heredity · 2026Article
- Host adaptation and genome evolution of the broad host range fungal rust pathogen, Austropuccinia psidii.G3 (Bethesda, Md.) · 2026Article
- High-quality draft genome assembly and functional annotation ofFrontiers in plant science · 2026Article
- Genetic structure and historical gene flow in Araucaria angustifolia populations across two Brazilian regions.Heredity · 2025Article
- An optimized DNA extraction protocol for reliable PCR-based detection and characterization of grapevine flavescence dorée phytoplasma.Plant methods · 2025Article
- Fungi of the family Psathyrellaceae are symbiotic partners of the mycoheterotrophic orchid Danxiaorchis yangii.Mycorrhiza · 2025Article
- A slow march along the South American Dry Diagonal: a multi-gene molecular phylogeny and biogeographical history of the peanut genus, Arachis L.Annals of botany · 2025Article
- The genome assembly of the duckweed fern, Azolla caroliniana.The Journal of heredity · 2025Article
63 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites. We therefore investigated the utility of a pre-wash step using a buffered sorbitol solution, prior to DNA extraction using a high salt CTAB extraction protocol, in a high throughput or miniprep setting. This pre-wash appears to remove interfering metabolites, such as polyphenols and polysaccharides, from tissue macerates. We also investigated the adaptability of the sorbitol pre-wash for RNA extraction using a lithium chloride-based protocol. The method was successfully applied to a variety of tissues, including leaf, cambium and fruit of diverse plant species including annual crops, forest and fruit trees, herbarium leaf material and lyophilized fungal mycelium. We consistently obtained good yields of high purity DNA or RNA in all species tested. The protocol has been validated for thousands of DNA samples by generating high data quality in dense SNP arrays. DNA extracted from Eucalyptus spp. leaf and cambium as well as mycelium from Trichoderma spp. was readily digested with restriction enzymes and performed consistently in AFLP assays. Scaled-up DNA extractions were also suitable for long read sequencing. Successful RNA quality control and good RNA-Seq data for Eucalyptus and cashew confirms the effectiveness of the sorbitol buffer pre-wash for high quality RNA extraction.
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