ArticlePloS one2018
Resveratrol enhances the clearance of mitochondrial damage by vitrification and improves the development of vitrified-warmed bovine embryos.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- The role of mitochondrial DNA copy number in spent culture medium in predicting outcomes of single blastocyst transfer.Journal of assisted reproduction and genetics · 2025Article
- Nuclear Genome-Encoded Mitochondrial OXPHOS Complex I Genes in Female Buffalo Show Tissue-Specific Differences.Molecular biotechnology · 2025Article
- Spermidine Supplementation Effectively Improves the Quality of Mouse Oocytes After Vitrification Freezing.Antioxidants (Basel, Switzerland) · 2025Article
- Reactive oxygen species attenuation improves the quality of vitrified-warmed bovine embryos.Animal reproduction · 2025Article
- Transcriptomic Analysis of Vitrified-Warmed vs. Fresh Mouse Blastocysts: Cryo-Induced Physiological Mechanisms and Implantation Impact.International journal of molecular sciences · 2024Article
- Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse blastocysts.The Journal of reproduction and development · 2024Article
- Superior performance of biocomposite nanoparticles PLGA-RES in protecting oocytes against vitrification stimuli.Frontiers in bioengineering and biotechnology · 2024Article
- Current Advances in Bovine In Vitro Maturation and Embryo Production Using Different Antioxidants: A Review.Journal of developmental biology · 2023Review
- Article
- Antioxidant procyanidin B2 protects oocytes against cryoinjuries via mitochondria regulated cortical tension.Journal of animal science and biotechnology · 2022Article
- Vitrification of Pronuclear Zygotes Perturbs Porcine Zygotic Genome Activation.Animals : an open access journal from MDPI · 2022Article
- Astaxanthin Supplementation Improves the Subsequent Developmental Competence of Vitrified Porcine Zygotes.Frontiers in veterinary science · 2022Article
- Resveratrol enhanced mitochondrial recovery from cryopreservation-induced damages in oocytes and embryos.Reproductive medicine and biology · 2021Review
- Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos.The Journal of reproduction and development · 2021Article
- Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos.The Journal of reproduction and development · 2020Article
- Abundance of cell-free mitochondrial DNA in spent culture medium associated with morphokinetics and blastocyst collapse of expanded blastocysts.Reproductive medicine and biology · 2020Article
- Long-Term Phenotypic and Proteomic Changes Following Vitrified Embryo Transfer in the Rabbit Model.Animals : an open access journal from MDPI · 2020Article
- Cell-free DNA content in follicular fluid: A marker for the developmental ability of porcine oocytes.Reproductive medicine and biology · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study investigated the vitrification-induced deterioration of mitochondrial functions that may reduce the developmental ability of post-warming bovine embryos. In addition, the effect of supplementation of the culture medium with resveratrol on the mitochondrial functions and post-warming embryonic development was examined. Two days after in vitro fertilization, embryos with 8-12 cells (referred to hereafter as 8-cell embryos) were vitrified and warmed, followed by in vitro incubation for 5 days in a culture medium containing either the vehicle or 0.5 μM resveratrol. Vitrification reduced embryonic development until the blastocyst stage, reduced the ATP content of embryos, and impaired the mitochondrial genome integrity, as determined by real-time polymerase chain reaction. Although the total cell number and mitochondrial DNA copy number (Mt-number) of blastocysts were low in the vitrified embryos, the Mt-number per blastomere was similar among the blastocysts derived from fresh (non-vitrified) and vitrified-warmed embryos. Supplementation of the culture medium with resveratrol enhanced the post-warming embryonic development and reduced the Mt-number and reactive oxygen species level in blastocysts and blastomeres without affecting the ATP content. An increase in the content of cell-free mitochondrial DNA in the spent culture medium was observed following cultivation of embryos with resveratrol. These results suggested that vitrification induces mitochondrial damages and that resveratrol may enhance the development of post-warming embryos and activates the degeneration of damaged mitochondria, as indicated by the increase in the cell-free mitochondrial DNA content in the spent culture medium and the decrease in the Mt-number of blastocysts and blastomeres.
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