ArticlePLoS neglected tropical diseases2018
A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Reverse vaccinology-driven construction and bioinformatics validation of a multi-epitope vaccine against Brucella spp.Scientific reports · 2025Article
- An infant brucellosis meningitis caused byBiosafety and health · 2025Article
- Unraveling brucellosis: advances in pathogenesis, diagnostic strategies, therapeutic innovations, and public health perspectives.Frontiers in medicine · 2025Review
- Delay in the diagnosis of Brucella abortus bacteremia in a nonendemic country: a case report.BMC infectious diseases · 2024Article
- Iron-tannic acid nano-coating: A promising treatment approach for enhancingSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2024Article
- Combination ofFrontiers in microbiology · 2024Article
- VirulentmSphere · 2023Article
- Case Report: Acute Brucellosis Due to Brucella suis in a Brazilian Gold Miner Diagnosed in French Guiana.The American journal of tropical medicine and hygiene · 2023Article
- Review
- Novel Species of Brucella Causing Human Brucellosis, French Guiana.Emerging infectious diseases · 2023Article
- Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019-present).Veterinary world · 2022Review
- The role of the serum tube agglutination test in the monitoring of human brucellosis: evaluation of post-treatment SAT titers.Revista da Associacao Medica Brasileira (1992) · 2022Article
- Machine Learning Algorithms for Classification of MALDI-TOF MS Spectra from Phylogenetically Closely Related SpeciesMicroorganisms · 2022Article
- High-Resolution Melting PCR as Rapid Genotyping Tool forMicroorganisms · 2022Article
- Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview.Pathogens (Basel, Switzerland) · 2021Review
- Approaches for characterizing and tracking hospital-associated multidrug-resistant bacteria.Cellular and molecular life sciences : CMLS · 2021Review
- Identification and Antifungal Susceptibility Analysis ofFrontiers in microbiology · 2021Article
- rRNA Analysis Based on Long-Read High-Throughput Sequencing Reveals a More Accurate Diagnostic for the Bacterial Infection of Ascites.BioMed research international · 2021Article
- Protein Biomarker Identification for the Discrimination ofFrontiers in microbiology · 2021Article
- MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks.Scientific reports · 2020Article
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella are highly infectious bacterial pathogens responsible for a severely debilitating zoonosis called brucellosis. Half of the human population worldwide is considered to live at risk of exposure, mostly in the poorest rural areas of the world. Prompt diagnosis of brucellosis is essential to prevent complications and to control epidemiology outbreaks, but identification of Brucella isolates may be hampered by the lack of rapid and cost-effective methods. Nowadays, many clinical microbiology laboratories use Matrix-Assisted Laser Desorption Ionization-Time Of Flight mass spectrometry (MALDI-TOF MS) for routine identification. However, lack of reference spectra in the currently commercialized databases does not allow the identification of Brucella isolates. In this work, we constructed a Brucella MALDI-TOF MS reference database using VITEK MS. We generated 590 spectra from 84 different strains (including rare or atypical isolates) to cover this bacterial genus. We then applied a novel biomathematical approach to discriminate different species. This allowed accurate identification of Brucella isolates at the genus level with no misidentifications, in particular as the closely related and less pathogenic Ochrobactrum genus. The main zoonotic species (B. melitensis, B. abortus and B. suis) could also be identified at the species level with an accuracy of 100%, 92.9% and 100%, respectively. This MALDI-TOF reference database will be the first Brucella database validated for diagnostic and accessible to all VITEK MS users in routine. This will improve the diagnosis and control of brucellosis by allowing a rapid identification of these pathogens.
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