ArticleJournal of biomedical optics2018
Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy.
Article in Journal of biomedical optics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Flow orientation as a critical parameter in nanoscale membrane filtration for optimising small extracellular vesicle isolation.Nanoscale advances · 2026Article
- Artifact detection in fluorescence microscopy using convolutional autoencoder.Scientific reports · 2025Article
- Supramolecular Conductive Hydrogels With Homogeneous Ionic and Electronic Transport.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Deep learning-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Precision and Accuracy of Receptor Quantification on Synthetic and Biological Surfaces Using DNA-PAINT.ACS sensors · 2023Article
- Spectrally PAINTing a Single Chain Polymeric Nanoparticle at Super-Resolution.Journal of the American Chemical Society · 2022Article
- Super-resolution imaging of flat-mounted whole mouse cornea.Experimental eye research · 2021Article
- Single-molecule localization microscopy.Nature reviews. Methods primers · 2021Article
- RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction.Bioinformatics (Oxford, England) · 2020Article
- Super-Resolution Imaging of Self-Assembled Nanocarriers Using Quantitative Spectroscopic Analysis for Cluster Extraction.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Article
- Multicolor super-resolution imaging using spectroscopic single-molecule localization microscopy with optimal spectral dispersion.Applied optics · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The existence of fluorescent impurities has been a long-standing obstacle in single-molecule imaging, which results in sample misidentification and higher localization uncertainty. Spectroscopic single-molecule localization microscopy can record the full fluorescent spectrum of every stochastic single-molecule emission event. This capability allows us to quantify the spatial and spectral characteristics of fluorescent impurities introduced by sample preparation steps, based on which we developed a method to effectively separate fluorescent impurities from target molecules.
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Registered trials
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