ArticleCell proliferation2019
Comparison of different in vitro differentiation conditions for murine female germline stem cells.
Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Dual recombinases-mediated genetic tracing reveals no postnatal neo-oogenesis in mice.iScience · 2026Article
- Machine learning-based prediction of IVF/ICSI outcomes in male factor infertility highlighting couple-level BMI.Frontiers in endocrinology · 2026Article
- Untargeted metabolomics reveals homogeneity and heterogeneity between physiological and pathological ovarian aging.Journal of ovarian research · 2025Article
- Cadherin-18 loss in prospermatogonia and spermatogonial stem cells enhances cell adhesion through a compensatory mechanism.Zoological research · 2024Article
- Inhibition of EED activity enhances cell survival of female germline stem cell and improves the oocytes production during oogenesisOpen biology · 2023Article
- 2cChIP-seq and 2cMeDIP-seq: The Carrier-Assisted Methods for Epigenomic Profiling of Small Cell Numbers or Single Cells.International journal of molecular sciences · 2022Article
- Retinoic acid induced meiosis initiation in female germline stem cells by remodelling three-dimensional chromatin structure.Cell proliferation · 2022Article
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Non-neutral clonal selection and its potential role in mammalian germline stem cell dysfunction with advancing age.Frontiers in cell and developmental biology · 2022Review
- Article
- Serum- and Feeder-Free Culture of Juvenile Monkey Female Germline Stem Cells and Testosterone Regulation of their Self-Renewal.Stem cell reviews and reports · 2022Article
- Ubiquitin-Specific-Processing Protease 7 Regulates Female Germline Stem Cell Self-Renewal Through DNA Methylation.Stem cell reviews and reports · 2021Article
- In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded.Cells · 2020Review
- Integrated Glycosylation Patterns of Glycoproteins and DNA Methylation Landscapes in Mammalian Oogenesis and Preimplantation Embryo Development.Frontiers in cell and developmental biology · 2020Article
- GAS5/miR-21 Axis as a Potential Target to Rescue ZCL-082-Induced Autophagy of Female Germline Stem Cells In Vitro.Molecular therapy. Nucleic acids · 2019Article
- Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness.Stem cell research & therapy · 2019Article
- C89 Induces Autophagy of Female Germline Stem Cells via Inhibition of the PI3K-Akt Pathway In Vitro.Cells · 2019Article
- Comparison of different in vitro differentiation conditions for murine female germline stem cells.Cell proliferation · 2019Article
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Authors and funding
8 authors.
Funding
Abstract
objectivesIn vitro differentiation of oocytes from female germline stem cells (FGSCs) has exciting potential applications for reproductive medicine. Some researchers have attempted to reveal the in vitro differentiation capacity of FGSCs. However, no systematic comparative study of in vitro differentiation conditions has been performed for murine FGSCs (mFGSCs). MATERIALS AND
methodsmFGSCs line was cultured under five different conditions for in vitro differentiation. RT-PCR was performed to detect the expression of Oct4, Fragilis, Blimp1, Mvh, Scp3 and Zp3. Immunofluorescence was carried out to test the expression of Mvh, Fragilis and Zp3. Two-photon laser-scanning microscope was used to analyze nucleus-plasma ratio, and the proportion of chromatin of GV oocytes differentiated from mFGSCs in vitro (IVD-GVO), GV oocytes from in vivo (GVO) and mFGSCs.
resultsRT-PCR and immunofluorescence showed that mFGSC line expressed germ cell-specific markers, but not a meiosis-specific marker. By evaluating five different in vitro differentiation conditions, condition 5, which included a hanging drop procedure and co-culture of mFGSCs with granulosa cells, was shown to be optimal. mFGSCs could be successfully differentiated into germinal vesicle (GV) -stage oocytes under this condition. 3D observation revealed that both the nucleus-plasma ratio and proportion of chromatin were not significantly different between IVD-GVO and GVO.
conclusionWe evaluated five in vitro differentiation conditions for mFGSCs and successfully differentiate mFGSCs into GV-stage oocytes using a three-step differentiation process.
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