Evidence map›Paper›PMID 30333092›Full record

ArticleBiochemical and biophysical research communications2018

Nano-structural analysis of engrafted human induced pluripotent stem cell-derived cardiomyocytes in mouse hearts using a genetic-probe APEX2.

Takeshi Hatani, Shunsuke Funakoshi, Thomas J Deerinck, Eric A Bushong, Takeshi Kimura, Hiroshi Takeshima, Mark H Ellisman, Masahiko Hoshijima, Yoshinori Yoshida

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Takeshi HataniDepartment of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan; Center for iPS Cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
Shunsuke FunakoshiCenter for iPS Cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan.
Thomas J DeerinckCenter for Research in Biological Systems, University of California at San Diego, La Jolla, CA, 92093, USA; National Center for Microscopy and Imaging Research, University of California at San Diego, La Jolla, CA, 92093, USA.
Eric A BushongCenter for Research in Biological Systems, University of California at San Diego, La Jolla, CA, 92093, USA; National Center for Microscopy and Imaging Research, University of California at San Diego, La Jolla, CA, 92093, USA.
Takeshi KimuraDepartment of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan.
Hiroshi TakeshimaGraduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606-8501, Japan.
Mark H EllismanCenter for Research in Biological Systems, University of California at San Diego, La Jolla, CA, 92093, USA; National Center for Microscopy and Imaging Research, University of California at San Diego, La Jolla, CA, 92093, USA; Departments of Neurosciences and Bioengineering, University of California at San Diego, La Jolla, CA, 92093, USA.
Masahiko HoshijimaCenter for Research in Biological Systems, University of California at San Diego, La Jolla, CA, 92093, USA; Department of Medicine, University of California at San Diego, La Jolla, CA, 92093, USA. Electronic address: mhoshijima@gmail.com.
Yoshinori YoshidaCenter for iPS Cell Research and Application, Kyoto University, Kyoto, 606-8507, Japan. Electronic address: yoshinor@cira.kyoto-u.ac.jp.
Kyoto University · JPUniversity of California San Diego · US

Funding

IVEM and Image Analysis ResourceP41GM103412 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2012 to 2019
$17.9M
New Probe and Methods for Correlated LM & EMR01GM086197 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ADAMS, STEPHEN ROY, BOASSA, DANIELA · 2008 to 2020
$8.3M
Support of and Extensions to the Cell Image LibraryR01GM082949 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2007 to 2019
$4.1M
3D Reconstruction and Analysis of Alzheimer's Patient Biopsy Samples to Map and Quantify Hallmarks of Pathogenesis and VulnerabilityR01AG065549 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2020 to 2024
$4.1M
The National Center for Microscopy and Imaging Research, a Community-wide Scientific ResourceR24GM137200 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2020 to 2022
$3.0M
A Replacement 300 Kv IVEM for Meso-scale 3D Data Acquisition at the NCMIRS10RR023026 · NCRR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2007 to 2007
$2.0M
NCRR NIH HHS S10 RR023026NIA NIH HHS R01 AG065549NIGMS NIH HHS P41 GM103412NIGMS NIH HHS R01 GM082949NIGMS NIH HHS R01 GM086197NIGMS NIH HHS R24 GM137200
6 · The paper itself

Abstract

Many studies have shown the feasibility of in vivo cardiac transplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in animal experiments. However, nano-structural confirmation of the successful incorporation of the engrafted iPSC-CMs including electron microscopy (EM) has not been accomplished, partly because identification of graft cells in EM has proven to be difficult. Using APEX2, an engineered ascorbate peroxidase imaging tag, we successfully localized and analyzed the fine structure of sarcomeres and the excitation contraction machinery of iPSC-CMs 6 months after their engraftment in infarcted mouse hearts. APEX2 made iPSC-CMs visible in multiple imaging modalities including light microscopy, X-ray microscopic tomography, transmission EM, and scanning EM. EM tomography allowed assessment of the differentiation state of APEX2-positive iPSC-CMs and analysis of the fine structure of the sarcomeres including T-tubules and dyads.

Indexed as

AnimalsCell DifferentiationCell LineCell LineageDNA-(Apurinic or Apyrimidinic Site) LyaseHeartHumansInduced Pluripotent Stem CellsMaleMiceMolecular ProbesMyocardial InfarctionMyocardiumMyocytes, CardiacDNA-(Apurinic or Apyrimidinic Site) LyaseMolecular ProbesAPEX2Cardiac cell transplantationElectron microscopyiPSC-derived cardiomyocytesRegeneration therapy

Identifiers

PMID30333092
PMCPMC7478876
OpenAlexW2897407037

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.