ArticleBMC plant biology2018
A combined small RNA and transcriptome sequencing analysis reveal regulatory roles of miRNAs during anther development of Upland cotton carrying cytoplasmic male sterile Gossypium harknessii (D2) cytoplasm.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Heat stress-induced remodelling of lipid and microRNA networks in Brassica napus germinated pollen and pollen-derived small extracellular vesicles.Journal of experimental botany · 2026Article
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- Insights into the Early Steps of the Symbiotic Interaction between Soybean (Journal of agricultural and food chemistry · 2024Article
- Identification and characterization of circular RNAs involved in the fertility stability of cotton CMS-D2 restorer line under heat stress.BMC plant biology · 2024Article
- Heat-responsive microRNAs participate in regulating the pollen fertility stability of CMS-D2 restorer line under high-temperature stress.Biological research · 2023Article
- Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton.International journal of molecular sciences · 2023Article
- Development of mitochondrial simple sequence repeat markers to simultaneously distinguish cytoplasmic male sterile sources in cotton.Functional & integrative genomics · 2022Article
- Identification and analysis of oil candidate genes reveals the molecular basis of cottonseed oil accumulation in Gossypium hirsutum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Comparative transcriptome analysis of fiber and nonfiber tissues to identify the genes preferentially expressed in fiber development in Gossypium hirsutum.Scientific reports · 2021Article
- Identification and molecular characterization of miRNAs and their target genes associated with seed development through small RNA sequencing in chickpea.Functional & integrative genomics · 2021Article
- Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 are Involved in the Regulation of Male Sterility in PTGMS Rice.International journal of molecular sciences · 2021Article
- Article
- Profiling of MicroRNAs Involved in Mepiquat Chloride-Mediated Inhibition of Internode Elongation in Cotton (Frontiers in plant science · 2021Article
- GWAS Mediated Elucidation of Heterosis for Metric Traits in Cotton (Frontiers in plant science · 2021Article
- Article
- Comparative transcriptome analysis between inbred and hybrids reveals molecular insights into yield heterosis of upland cotton.BMC plant biology · 2020Article
- Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing.Molecular genetics and genomics : MGG · 2020Article
- Comparative transcriptome analysis of inbred lines and contrasting hybrids reveals overdominance mediate early biomass vigor in hybrid cotton.BMC genomics · 2020Article
- Genome-Wide Characterization and Analysis ofInternational journal of molecular sciences · 2020Article
- Integrated Methylome and Transcriptome Analysis between the CMS-D2 Line ZBA and Its Maintainer Line ZB in Upland Cotton.International journal of molecular sciences · 2019Article
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundCytoplasmic male sterility (CMS) in flowering plants is usually caused by incompatibility between mitochondrial and nuclear genomes, and can be restored by nuclear genes known as restorer-of-fertility (Rf). Although the CMS/Rf system is useful and convenient for economic production of commercial hybrid seed, the molecular mechanisms of CMS occurrence and fertility restoration in cotton are unclear.
resultsHere, a combined small RNA and transcriptome sequencing analysis was performed on floral buds at the meiosis stage in three-line hybrid cotton system, and differentially expressed microRNAs (DEMs) and their target genes were identified and further analyzed for a possible involvement in CMS and fertility restoration. Totally 10 and 30 differentially expressed miRNA-target gene pairs were identified in A-B and A-R comparison group, respectively. A putative regulatory network of CMS occurrence and fertility restoration-related miRNA-target pairs during anther development were then constructed. The RLM-RACE analysis showed that gra-miR7505b regulates a PPR gene (Gh_D05G3392) by cleaving precisely at the 643 nt and 748 nt sites. The further analysis indicated that the sequence variation in the binding regions of Gh_D05G3392 and Gh_D05G3356 may cause a lower cleavage efficiency of the PPR genes by miR7505b and miR7505 in R line, respectively, leading to the up-regulation of the PPR genes and fertility restoration. These results have established their genetic involvement in fertility restoration in the CMS-D2 system.
conclusionOur combined miRNA and transcriptome analysis in three-line hybrid cotton system provides new insights into the molecular mechanisms of CMS occurrence and fertility restoration, which will contribute to further hybrid breeding in cotton.
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